All Outputs (13)

Advances in Euglenoid Genomics: Unravelling the Fascinating Biology of a Complex Clade (2024)
Journal Article
Fields, O., Hammond, M., Xu, X., & O'Neill, E. (2024). Advances in Euglenoid Genomics: Unravelling the Fascinating Biology of a Complex Clade. Trends in Genetics, https://doi.org/10.1016/j.tig.2024.07.007

Euglenids have long been studied due to their unique physiology and versatile metabolism, providing underpinnings for much of our understanding of photosynthesis and biochemistry, and a growing opportunity in biotechnology. Until recently there has b... Read More about Advances in Euglenoid Genomics: Unravelling the Fascinating Biology of a Complex Clade.

Strategies for producing high value small molecules in microalgae (2024)
Journal Article
O'Neill, E., & Cagney, M. (2024). Strategies for producing high value small molecules in microalgae. Plant Physiology and Biochemistry, 214, Article 108942. https://doi.org/10.1016/j.plaphy.2024.108942

Eukaryotic microalgae are a diverse group of organisms that can be used for the sustainable production of a wide range of high value compounds, including lipids, flavours and colourants, bioplastics and cosmetics. Optimising total biomass production... Read More about Strategies for producing high value small molecules in microalgae.

The biomolecules of Euglena gracilis: Harnessing biology for natural solutions to future problems (2024)
Journal Article
Bedard, S., Roxborough, E., O'Neill, E., & Mangal, V. (2024). The biomolecules of Euglena gracilis: Harnessing biology for natural solutions to future problems. Protist, 175(4), Article 126044. https://doi.org/10.1016/j.protis.2024.126044

Over the past decade, the autotrophic and heterotrophic protist Euglena gracilis (E. gracilis) has gained popularity across the studies of environmental science, biosynthesis experiments, and nutritional substitutes. The unique physiology and versati... Read More about The biomolecules of Euglena gracilis: Harnessing biology for natural solutions to future problems.

microbeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data (2024)
Journal Article
Zuffa, S., Schmid, R., Bauermeister, A., Gomes, P. W. P., Caraballo-Rodriguez, A., Abiead, Y. E., …Dorrestein, P. (2024). microbeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data. Nature Microbiology, 9(2), 336-345. https://doi.org/10.1038/s41564-023-01575-9

microbeMASST, a taxonomically informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 microbial monocultures, users can search known... Read More about microbeMASST: a taxonomically informed mass spectrometry search tool for microbial metabolomics data.

A Taxonomically-informed Mass Spectrometry Search Tool for Microbial Metabolomics Data (2023)
Preprint / Working Paper
Zuffa, S., Schmid, R., Bauermeister, A., Gomes, P. W. P., Caraballo-Rodriguez, A. M., Abiead, Y. E., …Dorrestein, P. C. A Taxonomically-informed Mass Spectrometry Search Tool for Microbial Metabolomics Data

MicrobeMASST, a taxonomically-informed mass spectrometry (MS) search tool, tackles limited microbial metabolite annotation in untargeted metabolomics experiments. Leveraging a curated database of >60,000 microbial monocultures, users can search known... Read More about A Taxonomically-informed Mass Spectrometry Search Tool for Microbial Metabolomics Data.

Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach (2022)
Journal Article
O'Neill, E. C. (2023). Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach. FEMS Microbiology Letters, 370, Article fnac120. https://doi.org/10.1093/femsle/fnac120

Protein glycosylation, and in particular N-linked glycans, is a hallmark of eukaryotic cells and has been well-studied in mammalian cells and parasites. However, little research has been conducted to investigate the conservation and variation of prot... Read More about Glycosylated proteins in the protozoan alga Euglena gracilis: a proteomic approach.

Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world (2022)
Journal Article
Ebenezer, T. G. E., Low, R. S., O'Neill, E. C., Huang, I., DeSimone, A., Farrow, S. C., …Field, M. C. (2022). Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world. Biology Open, 11(11), Article 059561. https://doi.org/10.1242/bio.059561

Euglenoids (Euglenida) are unicellular flagellates possessing exceptionally wide geographical and ecological distribution. Euglenoids combine a biotechnological potential with a unique position in the eukaryotic tree of life. In large part these micr... Read More about Euglena International Network (EIN): Driving euglenoid biotechnology for the benefit of a challenged world.

Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis (2022)
Journal Article
Aldholmi, M., Ahmad, R., Carretero-Molina, D., Pérez-Victoria, I., Martín, J., Reyes, F., …Ganesan, A. (2022). Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis. Angewandte Chemie International Edition, 61(23), Article e202203175. https://doi.org/10.1002/anie.202203175

By limiting the nitrogen source to glutamic acid, we isolated cyclic peptides from Euglena gracilis containing asparagine and non-proteinogenic amino acids. Structure elucidation was accomplished through spectroscopic methods, mass spectrometry and c... Read More about Euglenatides, Potent Antiproliferative Cyclic Peptides Isolated from the Freshwater Photosynthetic Microalga Euglena gracilis.

A community resource for paired genomic and metabolomic data mining (2021)
Journal Article
Schorn, M. A., Verhoeven, S., Ridder, L., Huber, F., Acharya, D. D., Aksenov, A. A., …van der Hooft, J. J. J. (2021). A community resource for paired genomic and metabolomic data mining. Nature Chemical Biology, 17, 363-368. https://doi.org/10.1038/s41589-020-00724-z

Transcriptome, proteome and draft genome of Euglena gracilis (2019)
Journal Article
Ebenezer, T. E., Zoltner, M., Burrell, A., Nenarokova, A., Novák Vanclová, A. M. G., Prasad, B., …Field, M. C. (2019). Transcriptome, proteome and draft genome of Euglena gracilis. BMC Biology, 17(1), Article 11. https://doi.org/10.1186/s12915-019-0626-8

Background
Photosynthetic euglenids are major contributors to fresh water ecosystems. Euglena gracilis in particular has noted metabolic flexibility, reflected by an ability to thrive in a range of harsh environments. E. gracilis has been a popular... Read More about Transcriptome, proteome and draft genome of Euglena gracilis.

Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols (2016)
Journal Article
Crüsemann, M., O’Neill, E. C., Larson, C. B., Melnik, A. V., Floros, D. J., da Silva, R. R., …Moore, B. S. (2017). Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols. Journal of Natural Products, 80(3), 588-597. https://doi.org/10.1021/acs.jnatprod.6b00722

In order to expedite the rapid and efficient discovery and isolation of novel specialized metabolites, while minimizing the waste of resources on rediscovery of known compounds, it is crucial to develop efficient approaches for strain prioritization,... Read More about Prioritizing Natural Product Diversity in a Collection of 146 Bacterial Strains Based on Growth and Extraction Protocols.

Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining (2015)
Journal Article
Tang, X., Li, J., Millán-Aguiñaga, N., Zhang, J. J., O’Neill, E. C., Ugalde, J. A., …Moore, B. S. (2015). Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining. ACS Chemical Biology, 10(12), 2841-2849. https://doi.org/10.1021/acschembio.5b00658

Recent genome sequencing efforts have led to the rapid accumulation of uncharacterized or “orphaned” secondary metabolic biosynthesis gene clusters (BGCs) in public databases. This increase in DNA-sequenced big data has given rise to significant chal... Read More about Identification of Thiotetronic Acid Antibiotic Biosynthetic Pathways by Target-directed Genome Mining.

The transcriptome of Euglena gracilis reveals unexpected metabolic capabilities for carbohydrate and natural product biochemistry (2015)
Journal Article
O'Neill, E. C., Trick, M., Hill, L., Rejzek, M., Dusi, R. G., Hamilton, C. J., …Field, R. A. (2015). The transcriptome of Euglena gracilis reveals unexpected metabolic capabilities for carbohydrate and natural product biochemistry. Molecular BioSystems, 11(10), 2808-2820. https://doi.org/10.1039/c5mb00319a

Euglena gracilis is a highly complex alga belonging to the green plant line that shows characteristics of both plants and animals, while in evolutionary terms it is most closely related to the protozoan parasites Trypanosoma and Leishmania. This well... Read More about The transcriptome of Euglena gracilis reveals unexpected metabolic capabilities for carbohydrate and natural product biochemistry.