All Outputs (4)

Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair (2015)
Journal Article
Bolt, E. L., Jenkins, T., Russo, V. M., Ahmed, S., Cavey, J., & Cass, S. (2015). Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair. Bioscience Reports, https://doi.org/10.1042/BSR20150249

Using the ASKA (A Complete Set of E. coli K-12 ORF Archive) library for genome-wide screening of E. coli proteins we identified that expression of ygaQ and rpmG promotemitomycin C resistance (MMCR). YgaQ mediated MMCR was independent of homolog... Read More about Identification of Escherichia coli ygaQ and rpmG as novel mitomycin C resistance factors implicated in DNA repair.

Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity (2015)
Journal Article
Ivančić-Baće, I., Cass, S. D., Wearne, S. J., & Bolt, E. L. (2015). Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity. Nucleic Acids Research, 43(22), 10821-10830. https://doi.org/10.1093/nar/gkv1213

CRISPR-Cas is a prokaryotic immune system built from capture and integration of invader DNA into CRISPR (Clustered Regularly Interspaced Short Palindromic Repeats) loci, termed ‘Adaptation’, which is dependent on Cas1 and Cas2 proteins. In Escherichi... Read More about Different genome stability proteins underpin primed and naïve adaptation in E. coli CRISPR-Cas immunity.

Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition (2015)
Journal Article
Rollie, C., Schneider, S., Brinkmann, A. S., Bolt, E. L., & White, M. F. (2015). Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition. eLife, 4, Article e08716. https://doi.org/10.7554/elife.08716

© Rollie et al. The adaptive prokaryotic immune system CRISPR-Cas provides RNA-mediated protection from invading genetic elements. The fundamental basis of the system is the ability to capture small pieces of foreign DNA for incorporation into the ge... Read More about Intrinsic sequence specificity of the Cas1 integrase directs new spacer acquisition.

The role of Cas8 in type I CRISPR interference (2015)
Journal Article
Cass, S., Haas, K., Stoll, B., Alkhnbashi, O., Sharma, K., Urlaub, H., …Bolt, E. (2015). The role of Cas8 in type I CRISPR interference. Bioscience Reports, 35, Article e00197. https://doi.org/10.1042/BSR20150043

CRISPR (clustered regularly interspaced short palindromic repeat) systems provide bacteria and archaea with adapt- ive immunity to repel invasive genetic elements. Type I systems use ‘cascade’ [CRISPR-associated (Cas) complex for antiviral defence] r... Read More about The role of Cas8 in type I CRISPR interference.