A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans

Wilkinson, Daniel J.; Franchi, Martino V.; Brook, Matthew S.; Narici, Marco V.; Williams, John P.; Mitchell, William K.; Szewczyk, Nathaniel J.; Greenhaff, Paul L.; Atherton, Philip J.; Smith, Kenneth

doi:10.1152/ajpendo.00650.2013

All Outputs (45)

Skeletal muscle homeostasis and plasticity in youth and ageing: impact of nutrition and exercise (2015)
Journal Article
Brook, M. S., Wilkinson, D. J., Phillips, B. E., Perez-Schindler, J., Philp, A., Smith, K., & Atherton, P. J. (2016). Skeletal muscle homeostasis and plasticity in youth and ageing: impact of nutrition and exercise. Acta Physiologica, 216(1), 15-41. https://doi.org/10.1111/apha.12532

Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise (2015)
Journal Article
Bukhari, S. S., Phillips, B. E., Wilkinson, D. J., Limb, M. C., Rankin, D., Mitchell, W. K., …Atherton, P. J. (2015). Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise. AJP - Endocrinology and Metabolism, 308(12), E1056-E1065. https://doi.org/10.1152/ajpendo.00481.2014

© 2015 American Physiological Society. All Rights Reseved. Dysregulated anabolic responses to nutrition/exercise may contribute to sarcopenia; however, these characteristics are poorly defined in female populations. We determined the effects of two f... Read More about Intake of low-dose leucine-rich essential amino acids stimulates muscle anabolism equivalently to bolus whey protein in older women at rest and after exercise.

Skeletal muscle hypertrophy adaptations predominate in the early stages of resistance exercise training, matching deuterium oxide-derived measures of muscle protein synthesis and mechanistic target of rapamycin complex 1 signaling (2015)
Journal Article

Protein Carbonylation and Heat Shock Proteins in Human Skeletal Muscle: Relationships to Age and Sarcopenia (2014)
Journal Article

Aging is associated with a gradual loss of muscle mass termed sarcopenia, which has significant impact on quality-of-life. Because oxidative stress is proposed to negatively impact upon musculoskeletal aging, we investigated links between human aging... Read More about Protein Carbonylation and Heat Shock Proteins in Human Skeletal Muscle: Relationships to Age and Sarcopenia.

A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans (2013)
Journal Article
Wilkinson, D. J., Franchi, M. V., Brook, M. S., Narici, M. V., Williams, J. P., Mitchell, W. K., …Smith, K. (2014). A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans. AJP - Endocrinology and Metabolism, 306(5), Article E571-E579. https://doi.org/10.1152/ajpendo.00650.2013

Quantification of muscle protein synthesis (MPS) remains a cornerstone for understanding the control of muscle mass. Traditional [13C]amino acid tracer methodologies necessitate sustained bed rest and intravenous cannulation(s), restricting studies t... Read More about A validation of the application of D2O stable isotope tracer techniques for monitoring day-to-day changes in muscle protein subfraction synthesis in humans.