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All Outputs (12)

OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures (2023)
Preprint / Working Paper
Mendonca, T., Lis-Slimak, K., Matheson, A. B., Smith, M. G., Anane-Adjei, A. B., Cavanagh, R., …Wright, A. J. OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures

Biomechanical cues from the extracellular matrix (ECM) are essential for directing many cellular processes, from normal development and repair, to disease progression. To better understand cell-matrix interactions, we have developed a new instrument... Read More about OptoRheo: Simultaneous in situ micro-mechanical sensing and 3D imaging of live cell cultures.

Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging (2023)
Journal Article
Linke, F., Johnson, J. E. C., Kern, S., Bennett, C. D., Lourdusamy, A., Lea, D., …Coyle, B. (2023). Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging. Acta Neuropathologica Communications, 11(1), Article 6. https://doi.org/10.1186/s40478-022-01496-4

The most common malignant brain tumour in children, medulloblastoma (MB), is subdivided into four clinically relevant molecular subgroups, although targeted therapy options informed by understanding of different cellular features are lacking. Here, b... Read More about Identifying new biomarkers of aggressive Group 3 and SHH medulloblastoma using 3D hydrogel models, single cell RNA sequencing and 3D OrbiSIMS imaging.

Novel perspectives of sodium handling in type 2 diabetes mellitus (2022)
Journal Article
Hanson, P., Arkill, K. P., Merry, C. L., Hussain, M. S., Meersmann, T., Randeva, H. S., …Barber, T. M. (2022). Novel perspectives of sodium handling in type 2 diabetes mellitus. Expert Review of Endocrinology and Metabolism, 17(4), 333-341. https://doi.org/10.1080/17446651.2022.2092094

Introduction: As a key regulator of body water, sodium homeostasis forms an essential component of human physiology. Type 2 Diabetes Mellitus (T2D)-associated sodium overload stems from chronic renal retention of sodium, contributing toward the devel... Read More about Novel perspectives of sodium handling in type 2 diabetes mellitus.

High sensitivity analysis of nanogram quantities of glycosaminoglycans using ToF-SIMS (2021)
Journal Article
Hook, A. L., Hogwood, J., Gray, E., Mulloy, B., & Merry, C. L. (2021). High sensitivity analysis of nanogram quantities of glycosaminoglycans using ToF-SIMS. Communications Chemistry, 4, Article 67. https://doi.org/10.1038/s42004-021-00506-1

Glycosaminoglycans (GAGs) are important biopolymers that differ in the sequence of saccharide units and in post polymerization alterations at various positions, making these complex molecules challenging to analyse. Here we describe an approach that... Read More about High sensitivity analysis of nanogram quantities of glycosaminoglycans using ToF-SIMS.

Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities (2020)
Journal Article
Schneeberger, P., von Elsner, L., Barker, E. L., Meinecke, P., Marquardt, I., Alawi, M., …Kutsche, K. (2020). Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities. American Journal of Human Genetics, 107(6), 1044-1061. https://doi.org/10.1016/j.ajhg.2020.10.007

Heparan sulfate belongs to the group of glycosaminoglycans (GAGs), highly sulfated linear polysaccharides. Heparan sulfate 2-O-sulfotransferase 1 (HS2ST1) is one of several specialized enzymes required for heparan sulfate synthesis and catalyzes the... Read More about Bi-allelic Pathogenic Variants in HS2ST1 Cause a Syndrome Characterized by Developmental Delay and Corpus Callosum, Skeletal, and Renal Abnormalities.

Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease (2020)
Journal Article
Ashworth, J. C., Morgan, R. L., Lis-Slimak, K., Jones, S., Spence, K., Slater, C. E., …Merry, C. L. R. (2020). Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease. Journal of Visualized Experiments, 166, Article e61710. https://doi.org/10.3791/61710

There is a growing awareness that cells grown in 3D better model in vivo behavior than those grown in 2D. In this protocol, we describe a simple and tunable 3D hydrogel, suitable for culturing cells and tissue in a setting that matches their native e... Read More about Preparation of a User-Defined Peptide Gel for Controlled 3D Culture Models of Cancer and Disease.

3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome (2020)
Journal Article
Linke, F., Aldighieri, M., Lourdusamy, A., Grabowska, A. M., Stolnik, S., Kerr, I. D., …Coyle, B. (2021). 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome. Journal of Pathology, 253(3), 326-338. https://doi.org/10.1002/path.5591

© 2020 The Authors. The Journal of Pathology published by John Wiley & Sons, Ltd. on behalf of The Pathological Society of Great Britain and Ireland. Medulloblastoma (MB) is the most common malignant brain tumour in children and is subdivided into... Read More about 3D hydrogels reveal medulloblastoma subgroup differences and identify extracellular matrix subtypes that predict patient outcome.

Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro (2019)
Journal Article
Ashworth, J. C., Thompson, J. L., James, J. R., Slater, C. E., Pijuan-Galitó, S., Lis-Slimak, K., …Merry, C. L. (2020). Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro. Matrix Biology, 85-86, 15-33. https://doi.org/10.1016/j.matbio.2019.06.009

© 2019 Current materials used for in vitro 3D cell culture are often limited by their poor similarity to human tissue, batch-to-batch variability and complexity of composition and manufacture. Here, we present a “blank slate” culture environment base... Read More about Peptide gels of fully-defined composition and mechanics for probing cell-cell and cell-matrix interactions in vitro.

Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture (2016)
Journal Article

Reliable, scalable and time-efficient culture methods are required to fully realize the clinical and industrial applications of human pluripotent stem (hPS) cells. Here we present a completely defined, xeno-free medium that supports long-term propaga... Read More about Human serum-derived protein removes the need for coating in defined human pluripotent stem cell culture.

Heparan sulfate inhibits hematopoietic stem and progenitor cell migration and engraftment in mucopolysaccharidosis I. (2014)
Journal Article

Mucopolysaccharidosis I Hurler (MPSI-H) is a pediatric lysosomal storage disease caused by genetic deficiencies in IDUA, coding for ?-l-iduronidase. Idua?/? mice share similar clinical pathology with patients, including the accumulation of the undegr... Read More about Heparan sulfate inhibits hematopoietic stem and progenitor cell migration and engraftment in mucopolysaccharidosis I..

Novel cell lines isolated from mouse embryonic stem cells exhibiting de novo methylation of the E-cadherin promoter. (2014)
Journal Article

Mouse embryonic stem cells (mESCs) and epiblast stem cells represent the naïve and primed pluripotent states, respectively. These cells self‐renew via distinct signaling pathways and can transition between the two states in the presence of appropriat... Read More about Novel cell lines isolated from mouse embryonic stem cells exhibiting de novo methylation of the E-cadherin promoter..