All Outputs (126)

Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications (2012)
Journal Article

The amniotic membrane (AM) is considered as a natural cell culture substrate and has occasionally been exploited in regenerative medicine especially for ocular surface reconstruction and dermal wound healing applications. However, its use is limited... Read More about Tissue transglutaminase (TG-2) modified amniotic membrane: A novel scaffold for biomedical applications.

Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease (2012)
Journal Article

Dendritic cells (DCs) are part of the innate immune system with a key role in initiating and modulating T cell mediated immune responses. Coeliac disease is caused by inappropriate activation of such a response leading to small intestinal inflammatio... Read More about Tissue transglutaminase treatment leads to concentration-dependent changes in dendritic cell phenotype - implications for the role of transglutaminase in coeliac disease.

The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent (2012)
Journal Article

Allergens are initiators of both innate and adaptive immune responses. They are recognised at the site of entry by epithelial and dendritic cells (DCs), both of which activate innate inflammatory circuits that can collectively induce Th2 immune respo... Read More about The glycosylation pattern of common allergens: the recognition and uptake of Der p 1 by epithelial and dendritic cells is carbohydrate dependent.

Separation of dendritic and T cells using electrowetting and dielectrophoresis (2012)
Conference Proceeding

The research of immune cells is fundamental to many biological studies. Dendritic cells have the ability to induce a primary immune response in resting naive T cells. The aim of this work is to separate the activated T cells from dendritic cells on a... Read More about Separation of dendritic and T cells using electrowetting and dielectrophoresis.

Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite (2012)
Journal Article

Dendritic cells (DCs) have been shown to play a key role in the initiation and maintenance of immune responses to microbial pathogens as well as to allergens, but the exact mechanisms of their involvement in allergic responses and Th2 cell differenti... Read More about Retagging identifies dendritic cell-specific intercellular adhesion molecule-3 (ICAM3)-grabbing non-integrin (DC-SIGN) protein as a novel receptor for a major allergen from house dust mite.

Biomimetic tissues on a chip for drug discovery (2011)
Journal Article

Developing biologically relevant models of human tissues and organs is an important enabling step for disease modeling and drug discovery. Recent advances in tissue engineering, biomaterials and microfluidics have led to the development of microscale... Read More about Biomimetic tissues on a chip for drug discovery.

Development of a tissue engineered 3D immunocompetent model of the human upper respiratory tract (2011)
Journal Article

Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry (2011)
Journal Article

Uniform cellular distribution is a prerequisite to forming tissue within porous scaffolds, but the seeding process often results in preferential adhesion of cells at the periphery. We develop a vapour phase coating strategy which is readily applicabl... Read More about Uniform cell colonization of porous 3-D scaffolds achieved using radial control of surface chemistry.

Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity (2011)
Journal Article

Dendritic cells are professional antigen-presenting cells that are specialized in antigen uptake and presentation. Allergy to cat has increased substantially in recent years and has been shown to be positively associated with asthma.Wehave recently s... Read More about Recognition of the major cat allergen Fel d 1 through the cysteine-rich domain of the mannose receptor determines its allergenicity.

Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins (2010)
Journal Article

Surface Ig (sIg) of follicular lymphoma (FL) is vital for tumor cell survival. We found previously that the Ig in FL is unusual, because the variable region genes carry sequence motifs for N-glycan addition. These are introduced by somatic mutation a... Read More about Glycosylation of surface Ig creates a functional bridge between human follicular lymphoma and microenvironmental lectins.

Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy (2010)
Journal Article

Confocal Raman micro-spectroscopy (CRMS) was used to measure spectral images of immunological synapse formation between dendritic and T cells without using molecular labels or other invasive procedures. The purpose-built inverted CRMS instrument inte... Read More about Label-free molecular imaging of immunological synapses between dendritic and T cells by Raman micro-spectroscopy.

The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity (2010)
Journal Article

The mannose receptor (MR) is a C-type lectin expressed by dendritic cells (DCs). We have investigated the ability of MR to recognize glycosylated allergens. Using a gene silencing strategy, we have specifically inhibited the expression of MR on human... Read More about The mannose receptor mediates the uptake of diverse native allergens by dendritic cells and determines allergen-induced T cell polarization through modulation of IDO Activity.

Laminin and fibronectin treatment leads to generation of dendritic cells with superior endocytic capacity (2010)
Journal Article

Background: Sampling the microenvironment at sites of microbial exposure by dendritic cells (DC) and their subsequent interaction with T cells in the paracortical area of lymph nodes are key events for initiating immune responses. Most of our knowled... Read More about Laminin and fibronectin treatment leads to generation of dendritic cells with superior endocytic capacity.

Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen der p 1 (2009)
Journal Article

Background: Dendritic cells (DCs) are sentinels of the immune system and are known to play a key role in allergic responses. However, it is not clear how DCs that have been exposed to an allergen support Th2 type immune responses. It is possible that... Read More about Differential protein expression by dendritic cells from atopic and non-atopic individuals after stimulation by the major house dust mite allergen der p 1.

The molecular basis of allergenicity (2008)
Journal Article

Allergens are mostly innocuous antigens that elicit powerful T helper cell type 2 (Th2) responses leading to hyper-immunoglobulin E (IgE) production and allergy. Research carried out over several years has highlighted the possible role of the inheren... Read More about The molecular basis of allergenicity.

Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function (2008)
Journal Article

Dendritic cells are a major source of extracellular thiols needed for T cell activation, a process in which CD40-mediated stimulation plays a pivotal role. The Dermatophagoides pteronyssinus group 1 mite allergen (Der p 1) has previously been shown t... Read More about Allergen-driven suppression of thiol production by human dendritic cells and the effect of thiols on T cell function.

An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcεRI with FcγRIIb (2007)
Journal Article

Background: IgE binds to mast cells and basophils via its high-affinity receptor, FcεRI, and cross-linking of FcεRI-bound IgE molecules by allergen leads to the release of allergic mediators characteristic of type I hypersensitivity reactions. Previo... Read More about An immunoglobulin E-reactive chimeric human immunoglobulin G1 anti-idiotype inhibits basophil degranulation through cross-linking of FcεRI with FcγRIIb.

Elevated CD16 expression by monocytes from patients with tumor necrosis factor receptor-associated periodic syndrome (2007)
Journal Article

Objective. Tumor necrosis factor receptor-associated periodic syndrome (TRAPS) is an inherited autosomal-dominant autoinflammatory condition caused by mutations in the ectodomain of the 55-kd tumor necrosis factor (TNF) receptor superfamily 1A. Proin... Read More about Elevated CD16 expression by monocytes from patients with tumor necrosis factor receptor-associated periodic syndrome.

The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets (2007)
Journal Article

A disintegrin and metalloprotease (ADAM) proteins have wide ranging functions, including proteolytic cleavage of cell surface molecules, cell fusion, cell adhesion and intracellular signalling. Recent evidence suggests the involvement of ADAM8 in all... Read More about The detection of ADAM8 protein on cells of the human immune system and the demonstration of its expression on peripheral blood B cells, dendritic cells and monocyte subsets.

Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics (2007)
Journal Article

Background: Dendritic cells (DCs) are specialized antigen presenting cells that play a pivotal role in bridging innate and adaptive immune responses. Given the scarcity of peripheral blood myeloid dendritic cells (mDCs) investigators have used differ... Read More about Analysis of proteomic profiles and functional properties of human peripheral blood myeloid dendritic cells, monocyte-derived dendritic cells and the dendritic cell-like KG-1 cells reveals distinct characteristics.