All Outputs (93)

Controlling Incoming Macrophages to Implants: Responsiveness of Macrophages to Gelatin Micropatterns under M1/M2 Phenotype Defining Biochemical Stimulations (2017)
Journal Article
Dollinger, C., Ndreu-Halili, A., Uka, A., Singh, S., Sadam, H., Neuman, T., …Vrana, N. E. (2017). Controlling Incoming Macrophages to Implants: Responsiveness of Macrophages to Gelatin Micropatterns under M1/M2 Phenotype Defining Biochemical Stimulations. Advanced Biosystems, 1(6), Article 1700041. https://doi.org/10.1002/adbi.201700041

Adverse immune reactions to implanted devices can seriously hamper the efficacy of implants. Monocyte derived macrophages play a crucial role in both initiation and resolution of the inflammatory response toward foreign bodies. As the surface microt... Read More about Controlling Incoming Macrophages to Implants: Responsiveness of Macrophages to Gelatin Micropatterns under M1/M2 Phenotype Defining Biochemical Stimulations.

Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels (2017)
Journal Article
Dollinger, C., Ciftci, S., Knopf-Marques, H., Ghaemmaghami, A. M., Debry, C., Barthes, J., & Vrana, N. E. (2018). Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels. Journal of Tissue Engineering and Regenerative Medicine, 12(2), 330-340. https://doi.org/10.1002/term.2458

The success of tissue engineering strategy is strongly related to the inflammatory response, mainly through the activity of macrophages that are key cells in initial immune response to implants. For engineered tissues, the presence of resident macrop... Read More about Incorporation of resident macrophages in engineered tissues: Multiple cell type response to microenvironment controlled macrophage-laden gelatine hydrogels.

Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation (2017)
Journal Article
Riabov, V., Salazar, F., Htwe, S. S., Gudima, A., Schmuttermaier, C., Barthes, J., …Kzhyshkowska, J. (2017). Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation. Acta Biomaterialia, 53, 389-398. https://doi.org/10.1016/j.actbio.2017.01.071

The immediate tissue microenvironment of implanted biomedical devices and engineered tissues is highly influential on their long term fate and efficacy. The creation of a long-term anti-inflammatory microenvironment around implants and artificial tis... Read More about Generation of anti-inflammatory macrophages for implants and regenerative medicine using self-standing release systems with a phenotype-fixing cytokine cocktail formulation.

Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures (2017)
Journal Article
Pokorny, M., Klemes, J., Zidek, O., Dollinger, C., Ozcebe, G., Singh, S., …Vrana, N. E. (in press). Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures. Biomedical Physics and Engineering Express, 3(1), https://doi.org/10.1088/2057-1976/3/1/015002

Metallic implants are widely used in orthopaedic and orthodontic applications. However, generally surface treatment of the metallic surfaces is necessary to render them more biologically active. Herein, we describe a direct write printing method to m... Read More about Electrohydrodynamic printing as a method to micropattern large titanium implant surfaces with photocrosslinkable structures.

A paper-based in vitro model for on-chip investigation of the human respiratory system (2016)
Journal Article
Rahimi, R., Htwe, S. S., Ochoa, M., Donaldson, A., Zieger, M., Sood, R., …Ziaie, B. (in press). A paper-based in vitro model for on-chip investigation of the human respiratory system. Lab on a Chip, 16(22), https://doi.org/10.1039/C6LC00866F

Culturing cells at the air–liquid interface (ALI) is essential for creating functional in vitro models of lung tissues. We present the use of direct-patterned laser-treated hydrophobic paper as an effective semi-permeable membrane, ideal for ALI cell... Read More about A paper-based in vitro model for on-chip investigation of the human respiratory system.

Effective antigen presentation to helper T cells by human eosinophils (2016)
Journal Article
Farhan, R. K., Vickers, M. A., Ghaemmaghami, A. M., Hall, A. M., Barker, R. N., & Walsh, G. M. (in press). Effective antigen presentation to helper T cells by human eosinophils. Immunology, https://doi.org/10.1111/imm.12658

Although eosinophils are inflammatory cells, there is increasing attention on their immunomodulatory roles. For example, murine eosinophils can present antigen to CD4+ T helper (Th) cells, but it remains unclear whether human eosinophils also have th... Read More about Effective antigen presentation to helper T cells by human eosinophils.

The impact of surface chemistry modification on macrophage polarisation (2016)
Journal Article
Rostam, H., Singh, S., Salazar, F., Magennis, P., Hook, A. L., Singh, T., …Ghaemmaghami, A. M. (2016). The impact of surface chemistry modification on macrophage polarisation. Immunobiology, 221(11), 1237-1246. https://doi.org/10.1016/j.imbio.2016.06.010

Macrophages are innate immune cells that have a central role in combating infection and maintaining tissue homeostasis. They exhibit remarkable plasticity in response to environmental cues. At either end of a broad activation spectrum are pro-inflamm... Read More about The impact of surface chemistry modification on macrophage polarisation.

Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings (2016)
Journal Article
Knopf-Marques, H., Singh, S., Htwe, S. S., Wolfova, L., Buffa, R., Bacharouche, J., …Lavalle, P. (2016). Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings. Biomacromolecules, 17(6), 2189-2198. https://doi.org/10.1021/acs.biomac.6b00429

This study aims to design an optimal polyelectrolyte multilayer film of poly-L-lysine (PLL) and hyaluronic acid(HA) as an anti-inflammatory cytokine release system in order to decrease the implant failure due to any immune reactions. The chemical mod... Read More about Immunomodulation with self-crosslinked polyelectrolyte multilayer-based coatings.

Engineering Immunomodulatory Biomaterials To Tune the Inflammatory Response (2016)
Journal Article
Vishwakarma, A., Bhise, N. S., Evangelista, M. B., Rouwkema, J., Dokmeci, M. R., Ghaemmaghami, A. M., …Khademhosseini, A. (2016). Engineering Immunomodulatory Biomaterials To Tune the Inflammatory Response. Trends in Biotechnology, 34(6), 470-482. https://doi.org/10.1016/j.tibtech.2016.03.009

Current state-of-the-art biomedical implants and tissue engineering methods promise technologies to improve or even restore the function of diseased organs. However, one of the biggest challenges to clinical success is the lack of functional integrat... Read More about Engineering Immunomodulatory Biomaterials To Tune the Inflammatory Response.

Delivery strategies to control inflammatory response: Modulating M1–M2 polarization in tissue engineering applications (2016)
Journal Article
Alvarez, M. M., Liu, J. C., Trujillo-de Santiago, G., Cha, B. H., Vishwakarma, A., Ghaemmaghami, A. M., & Khademhosseini, A. (2016). Delivery strategies to control inflammatory response: Modulating M1–M2 polarization in tissue engineering applications. Journal of Controlled Release, 240, 349-363. https://doi.org/10.1016/j.jconrel.2016.01.026

Macrophages are key players in many physiological scenarios including tissue homeostasis. In response to injury, typically the balance between macrophage sub-populations shifts from an M1 phenotype (pro-inflammatory) to an M2 phenotype (anti-inflamma... Read More about Delivery strategies to control inflammatory response: Modulating M1–M2 polarization in tissue engineering applications.

Investigating NF-kappa B signaling in lung fibroblasts in 2D and 3D culture systems (2015)
Journal Article

Adapting the electrospinning process to provide three unique environments for a tri-layered in vitro model of the airway wall (2015)
Journal Article

Electrospinning is a highly adaptable method producing porous 3D fibrous scaffolds that can be exploited in in vitro cell culture. Alterations to intrinsic parameters within the process allow a high degree of control over scaffold characteristics inc... Read More about Adapting the electrospinning process to provide three unique environments for a tri-layered in vitro model of the airway wall.

TLR2 stimulation regulates the balance between regulatory T cell and Th17 function: A novel mechanism of reduced regulatory T cell function in multiple sclerosis (2015)
Journal Article
Nyirenda, M. H., Morandi, E., Vinkemeier, U., Constantin-Teodosiu, D., Drinkwater, S., Mee, M., …Gran, B. (2015). TLR2 stimulation regulates the balance between regulatory T cell and Th17 function: A novel mechanism of reduced regulatory T cell function in multiple sclerosis. Journal of Immunology, 194(12), 5761-5774. https://doi.org/10.4049/jimmunol.1400472

CD4+ CD25hi FOXP3+ regulatory T cells (Tregs) maintain tolerance to self-Ags. Their defective function is involved in the pathogenesis of multiple sclerosis (MS), an inflammatory demyelinating disease of the CNS. However, the mechanisms of such defec... Read More about TLR2 stimulation regulates the balance between regulatory T cell and Th17 function: A novel mechanism of reduced regulatory T cell function in multiple sclerosis.

TLR2 Stimulation Regulates the Balance between Regulatory T Cell and Th17 Function: A Novel Mechanism of Reduced Regulatory T Cell Function in Multiple Sclerosis (2015)
Journal Article
Nyirenda, M. H., Morandi, E., Vinkemeier, U., Constantin-Teodosiu, D., Drinkwater, S., Mee, M., …Gran, B. (2015). TLR2 Stimulation Regulates the Balance between Regulatory T Cell and Th17 Function: A Novel Mechanism of Reduced Regulatory T Cell Function in Multiple Sclerosis. Journal of Immunology, 194(12), 5761-5774. https://doi.org/10.4049/%E2%80%8Bjimmunol.1400472

CD4+CD25hi FOXP3+ regulatory T cells (Tregs) maintain tolerance to self-Ags. Their defective function is involved in the pathogenesis of multiple sclerosis (MS), an inflammatory demyelinating disease of the CNS. However, the mechanisms of such defect... Read More about TLR2 Stimulation Regulates the Balance between Regulatory T Cell and Th17 Function: A Novel Mechanism of Reduced Regulatory T Cell Function in Multiple Sclerosis.

A Janus-paper PDMS platform for air-liquid interface cell culture applications (2015)
Journal Article
Rahimi, R., Ochoa, M., Donaldson, A., Parupudi, T., Dokmeci, M. R., Khademhosseini, A., …Ziaie, B. (2015). A Janus-paper PDMS platform for air-liquid interface cell culture applications. Journal of Micromechanics and Microengineering, 25(5), Article 055015. https://doi.org/10.1088/0960-1317/25/5/055015

© 2015 IOP Publishing Ltd. A commercially available Janus paper with one hydrophobic (polyethylene-coated) face and a hygroscopic/hydrophilic one is irreversibly bonded to a polydimethylsiloxane (PDMS) substrate incorporating microfluidic channels vi... Read More about A Janus-paper PDMS platform for air-liquid interface cell culture applications.

Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering (2015)
Journal Article
Zhao, X., Lang, Q., Yildirimer, L., Lin, Z. Y., Cui, W., Annabi, N., …Khademhosseini, A. (2016). Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering. Advanced Healthcare Materials, 5(1), 108-118. https://doi.org/10.1002/adhm.201500005

Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insu... Read More about Photocrosslinkable Gelatin Hydrogel for Epidermal Tissue Engineering.

Microfluidics for advanced drug delivery systems (2015)
Journal Article
Riahi, R., Tamayol, A., Shaegh, S. A. M., Ghaemmaghami, A. M., Dokmeci, M. R., & Khademshosseini, A. (2015). Microfluidics for advanced drug delivery systems. Current Opinion in Chemical Engineering, 7, 101-112. https://doi.org/10.1016/j.coche.2014.12.001

©2015 Elsevier Ltd. All rights reserved. Considerable efforts have been devoted toward developing effective drug delivery methods. Microfluidic systems, with their capability for precise handling and transport of small liquid quantities, have emerged... Read More about Microfluidics for advanced drug delivery systems.

Impact of surface chemistry and topography on the function of antigen presenting cells (2015)
Journal Article
Rostam, H. M., Singh, S., Vrana, N. E., Alexander, M. R., & Ghaemmaghami, A. M. (2015). Impact of surface chemistry and topography on the function of antigen presenting cells. Biomaterials Science, 3(3), 424-441. https://doi.org/10.1039/c4bm00375f

Antigen presenting cells (APCs) such as macrophages and dendritic cells (DCs) play a crucial role in orchestrating immune responses against foreign materials. The activation status of APCs can determine the outcome of an immune response following imp... Read More about Impact of surface chemistry and topography on the function of antigen presenting cells.

Surgical materials: Current challenges and nano-enabled solutions (2014)
Journal Article
Annabi, N., Tamayol, A., Shin, S. R., Ghaemmaghami, A. M., Peppas, N. A., & Khademhosseini, A. (2014). Surgical materials: Current challenges and nano-enabled solutions. Nano Today, 9(5), 574-589. https://doi.org/10.1016/j.nantod.2014.09.006

© 2014 Elsevier Ltd. All rights reserved. Surgical adhesive biomaterials have emerged as substitutes to sutures and staples in many clinical applications. Nano-enabled materials containing nanoparticles or having a distinct nanotopography have been u... Read More about Surgical materials: Current challenges and nano-enabled solutions.

Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds (2014)
Journal Article
Morris, G., Bridge, J., Eltboli, O., Lewis, M., Knox, A., Aylott, J. W., …Rose, F. R. (2014). Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds. AJP - Lung Cellular and Molecular Physiology, 307(1), Article L38-L47. https://doi.org/10.1152/ajplung.00318.2013

Human airway smooth muscle (HASM) contraction plays a central role in regulating airway resistance in both healthy and asthmatic bronchioles. In vitro studies that investigate the intricate mechanisms that regulate this contractile process are predom... Read More about Human airway smooth muscle maintain in situ cell orientation and phenotype when cultured on aligned electrospun scaffolds.