All Outputs (7)

High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis-Tandem Mass Spectrometry (LESA-MS/MS) (2025)
Journal Article
Meurs, J., Nasir, A., Figueredo, G. P., Burroughs, L., Abdelrazig, S. A., Denning, C., Winkler, D. A., Barrett, D. A., Kim, D. H., & Alexander, M. R. (2025). High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis-Tandem Mass Spectrometry (LESA-MS/MS). Analytical Chemistry, 97(24), 12776-12785. https://doi.org/10.1021/acs.analchem.5c01636

Biomaterials play an important role in medicine from contact lenses to joint replacements. High-throughput screening coupled with machine learning has identified synthetic polymers that prevent bacterial biofilm formation, prevent fungal cell attachm... Read More about High-Throughput Analysis of Protein Adsorption to a Large Library of Polymers Using Liquid Extraction Surface Analysis-Tandem Mass Spectrometry (LESA-MS/MS).

Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy (2024)
Journal Article
Owen, R., Nasir, A., H. Amer, M., Nie, C., Xue, X., Burroughs, L., Denning, C., D. Wildman, R., A. Khan, F., R. Alexander, M., & R. A. J. Rose, F. (2025). Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy. Advanced Intelligent Systems, 7(5), Article 2400573. https://doi.org/10.1002/aisy.202400573

High-throughput screening (HTS) can be used when ab initio information is unavailable for rational design of new materials, generating data on properties such as chemistry and topography that control cell behavior. Biomaterial screens are typically f... Read More about Computer Vision for Substrate Detection in High-Throughput Biomaterial Screens Using Bright-Field Microscopy.

Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes (2024)
Journal Article
Raniga, K., Nasir, A., Vo, N. T., Vaidyanathan, R., Dickerson, S., Hilcove, S., Mosqueira, D., Mirams, G. R., Clements, P., Hicks, R., Pointon, A., Stebbeds, W., Francis, J., & Denning, C. (2024). Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes. Cell Stem Cell, 31(3), 292-311. https://doi.org/10.1016/j.stem.2024.01.007

Advances in hiPSC isolation and reprogramming and hPSC-CM differentiation have prompted their therapeutic application and utilization for evaluating potential cardiovascular safety liabilities. In this perspective, we showcase key efforts toward the... Read More about Strengthening cardiac therapy pipelines using human pluripotent stem cell-derived cardiomyocytes.

Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes (2024)
Journal Article
Johnson, B. B., Cosson, M.-V., Tsansizi, L. I., Holmes, T. L., Gilmore, T., Hampton, K., Song, O.-R., Vo, N. T. N., Nasir, A., Chabronova, A., Denning, C., Peffers, M. J., Merry, C. L. R., Whitelock, J., Troeberg, L., Rushworth, S. A., Bernardo, A. S., & Smith, J. G. W. (2024). Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes. Cell Reports, 43(1), Article 113668. https://doi.org/10.1016/j.celrep.2023.113668

Perlecan (HSPG2), a heparan sulfate proteoglycan similar to agrin, is key for extracellular matrix (ECM) maturation and stabilization. Although crucial for cardiac development, its role remains elusive. We show that perlecan expression increases as c... Read More about Perlecan (HSPG2) promotes structural, contractile, and metabolic development of human cardiomyocytes.

ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma (2021)
Journal Article
Nasir, A., Cardall, A., Othman, R. T., Nicolaou, N., Lourdusamy, A., Linke, F., Onion, D., Ryzhova, M., Cameron, H., Valente, C., Korshunov, A., Pfister, S. M., Grabowska, A. M., Kerr, I. D., & Coyle, B. (2021). ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma. Neuro-Oncology Advances, 3(1), https://doi.org/10.1093/noajnl/vdab030

Background: Therapeutic intervention in metastatic medulloblastoma is dependent upon elucidating the underlying metastatic mechanism. We investigated whether an epithelialmesenchymal transition (EMT)-like pathway could drive medulloblastoma metastasi... Read More about ABCB1 inhibition provides a novel therapeutic target to block TWIST1-induced migration in medulloblastoma.

Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion (2020)
Journal Article
Nasir, A., Thorpe, J., Burroughs, L., Meurs, J., Pijuan‐Galito, S., Irvine, D. J., Alexander, M. R., & Denning, C. (2020). Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion. Advanced Healthcare Materials, 10(6), Article 2001448. https://doi.org/10.1002/adhm.202001448

Human pluripotent stem cells (hPSCs) can be expanded and differentiated in vitro into almost any adult tissue cell type, and thus have great potential as a source for cell therapies with biomedical application. In this study, a fully-defined polymer... Read More about Discovery of a Novel Polymer for Xeno-Free, Long-Term Culture of Human Pluripotent Stem Cell Expansion.

Polymer microparticles with defined surface chemistry and topography mediate the formation of stem cell aggregates and cardiomyocyte function (2019)
Journal Article
Alvarez-Paino, M., Amer, M. H., Nasir, A., Cuzzucoli Crucitti, V., Thorpe, J., Burroughs, L., Needham, D., Denning, C., Alexander, M. R., Alexander, C., & Rose, F. (2019). Polymer microparticles with defined surface chemistry and topography mediate the formation of stem cell aggregates and cardiomyocyte function. ACS Applied Materials and Interfaces, 11(38), 34560-34574. https://doi.org/10.1021/acsami.9b04769

Surface-functionalized microparticles are relevant to fields spanning engineering and biomedicine, with uses ranging from cell culture to advanced cell delivery. Varying topographies of biomaterial surfaces are also being investigated as mediators of... Read More about Polymer microparticles with defined surface chemistry and topography mediate the formation of stem cell aggregates and cardiomyocyte function.