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Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro

Bridge, Jack Christopher; Amer, Mahetab H.; Morris, Gavin E.; Martin, N.R.W.; Player, Darren J.; Knox, Alan J.; Aylott, Jonathan W.; Lewis, Mark P.; Rose, Felicity R.A.J.

Authors

Jack Christopher Bridge jack_bridge@hotmail.co.uk

Mahetab H. Amer mahetab.amer@hotmail.com

Gavin E. Morris

N.R.W. Martin

Darren J. Player

Alan J. Knox

Mark P. Lewis

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FELICITY ROSE FELICITY.ROSE@NOTTINGHAM.AC.UK
Professor of Biomaterials and Tissue Engineering



Abstract

Contractile dysfunction of smooth muscle (SM) is a feature of chronic cardiovascular, respiratory and gastro-intestinal diseases. Owing to the low availability of human ex vivo tissue for the assessment of SM contractile function, the aim of this study was to develop a novel in vitro SM model that possesses the ability to contract, and a method to measure its contractility. A range of electrospun scaffolds were produced from crosslinked gelatin and methacrylated gelatin (GelMA), generating highly aligned scaffolds with average fibre diameters ranging from 200 nm to several micrometres. Young's moduli of the scaffolds ranged from 1x105 to 1x107 Pa. Primary aortic smooth muscle cells (AoSMCs; rat) cells readily adhered to and proliferated on the fibrous scaffolds for up to 10 days. They formed highly aligned populations following the topographical cues of the aligned scaffolds and stained positive for SM markers, indicating a contractile phenotype. Cell-seeded GelMA scaffolds were able, upon stimulation with uridine 5'-triphosphate (UTP), to contract and their attachment to a force transducer allowed the force of contraction to be measured. Hence, these electrospun GelMA fibres can be used as biomimetic scaffolds for SM cell culture and in vitro model development, and enables the contractile forces generated by the aligned three-dimensional sheet of cells to be directly measured. This will supplement in vitro drug screening tools and facilitate discovery of disease mechanisms.

Citation

Bridge, J. C., Amer, M. H., Morris, G. E., Martin, N., Player, D. J., Knox, A. J., …Rose, F. R. (2018). Electrospun gelatin-based scaffolds as a novel 3D platform to study the function of contractile smooth muscle cells in vitro. Biomedical Physics and Engineering Express, 4(4), https://doi.org/10.1088/2057-1976/aace8f

Journal Article Type Article
Acceptance Date Jun 22, 2018
Online Publication Date Jun 22, 2018
Publication Date Jul 31, 2018
Deposit Date Jun 25, 2018
Publicly Available Date Jun 25, 2018
Journal Biomedical Physics & Engineering Express
Electronic ISSN 2057-1976
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 4
Issue 4
Article Number 045039
DOI https://doi.org/10.1088/2057-1976/aace8f
Keywords Electrospinning; 3D cell culture; Contractile; Smooth muscle; Tissue engineering; In vitro model; GelMA
Public URL http://eprints.nottingham.ac.uk/id/eprint/52590
Publisher URL http://dx.doi.org/10.1088/2057-1976/aace8f
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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