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Fibre sub-type specific conduction reveals metabolic function in mouse sciatic nerve

Rich, Laura; Brown, Angus M.


Laura Rich


The stimulus evoked compound action potential (CAP), recorded using suction electrodes, provides an index of the relative number of conducting axons within a nerve trunk. As such the CAP has been used to elucidate the diverse mechanisms of injury resulting from a variety of metabolic insults to central nervous white matter, whilst also providing a model with which to assess the benefits of clinically relevant neuro-protective strategies. In addition the technique lends itself to the study of metabolic cell-to-cell signalling that occurs between glial cells and neurones, and to exploring the ability of non-glucose substrates to support axon conduction. Although peripheral nerves are sensitive to metabolic insult and are susceptible to diabetic neuropathy, there is a lack of fundamental information regarding peripheral nerve metabolism. A confounding factor in such studies is the extended duration demanded by the experimental protocol, requiring stable recording for periods of many hours. We describe a method that allows us to record simultaneously the stimulus evoked CAPs from A and C fibres from mouse sciatic nerve, and demonstrate its utility as applied to investigations into fibre sub-type substrate use. Our results suggest that C fibres directly take up and metabolise fructose, whereas A fibre conduction is supported by fructose-derived lactate, implying there exist unique metabolic profiles in neighbouring fibre sub-types present within the same nerve trunk.


Rich, L., & Brown, A. M. (2018). Fibre sub-type specific conduction reveals metabolic function in mouse sciatic nerve. Journal of Physiology, 596(10),

Journal Article Type Article
Acceptance Date Mar 1, 2018
Online Publication Date Mar 8, 2018
Publication Date May 15, 2018
Deposit Date Jun 20, 2018
Publicly Available Date Mar 9, 2019
Journal Journal of Physiology
Print ISSN 0022-3751
Electronic ISSN 1469-7793
Publisher Wiley
Peer Reviewed Peer Reviewed
Volume 596
Issue 10
Public URL
Publisher URL


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