Anaerobic radical enzymes for biotechnology

Abstract

Enzymes that proceed through radical intermediates have a rich chemistry that includes functionalisation of otherwise unreactive carbon atoms, carbon-skeleton rearrangements, aromatic reductions, and unusual eliminations. Especially under anaerobic conditions, organisms have developed a wide range of approaches for managing these transformations that can be exploited to generate new biological routes towards both bulk and specialty chemicals. These routes are often either much more direct or allow access to molecules that are inaccessible through standard (bio)chemical approaches. This review gives an overview of some of the key enzymes in this area: benzoyl-CoA reductases (that effect the enzymatic Birch reduction), ketyl radical dehydratases, coenzyme B12-dependant enzymes, glycyl radical enzymes, and radical SAM (AdoMet radical) enzymes. These enzymes are discussed alongside biotechnological applications, highlighting the wide range of actual and potential uses. With the increased diversity in biotechnological approaches to obtaining these enzymes and information about them, even more of these amazing enzymes can be expected to find application in industrial processes.

Citation

Jäger, C. M., & Croft, A. K. (2018). Anaerobic radical enzymes for biotechnology. ChemBioEng Reviews, 5(3), 143-162. https://doi.org/10.1002/cben.201800003

• Journal Article Type: Article
• Acceptance Date: Mar 26, 2018
• Online Publication Date: Apr 26, 2018
• Publication Date: Jun 30, 2018
• Deposit Date: Apr 23, 2018
• Publicly Available Date: Apr 27, 2019
• Journal: ChemBioEng Reviews
• Electronic ISSN: 2196-9744
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 5
• Issue: 3
• Pages: 143-162
• DOI: https://doi.org/10.1002/cben.201800003
• Keywords: Radicals, Enzymes, Catalysis, Biotechnology, Anaerobic reactions
• Public URL: https://nottingham-repository.worktribe.com/output/928719
• Publisher URL: https://onlinelibrary.wiley.com/doi/abs/10.1002/cben.201800003
• Additional Information: This is the peer reviewed version of the following article: Jäger, C. M. and Croft, A. K. (2018), Anaerobic Radical Enzymes for Biotechnology. CBEN. doi:10.1002/cben.201800003 which has been published in final form at https://onlinelibrary.wiley.com/doi/abs/10.1002/cben.201800003 This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.