Erkin Kuru
Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation
Kuru, Erkin; Lambert, Carey; Jonathon, Rittichier; Till, Rob; Ducret, Adrien; Derouaux, Adeline; Gray, Joe; Biboy, Jacob; Vollmer, Waldemar; VanNieuwenhze, Michael; Brun, Yves; Sockett, Liz
Authors
Dr CAREY LAMBERT carey.lambert@nottingham.ac.uk
Research Fellow
Rittichier Jonathon
Rob Till
Adrien Ducret
Adeline Derouaux
Joe Gray
Jacob Biboy
Waldemar Vollmer
Michael VanNieuwenhze
Yves Brun
Liz Sockett
Abstract
Modification of essential bacterial peptidoglycan (PG) containing cell walls can lead to antibiotic resistance, for example β-lactam resistance by L,D-transpeptidase activities. Predatory Bdellovibrio bacteriovorus are naturally antibacterial and combat infections by traversing, modifying and finally destroying walls of Gram-negative prey bacteria, modifying their own PG as they grow inside prey. Historically, these multi-enzymatic processes on two similar PG walls have proved challenging to elucidate. Here, with a PG labelling approach utilizing timed pulses of multiple fluorescent D-amino acids (FDAAs), we illuminate dynamic changes that predator and prey walls go through during the different phases of bacteria:bacteria invasion. We show formation of a reinforced circular port-hole in the prey wall; L,D-transpeptidaseBd mediated D-amino acid modifications strengthening prey PG during Bdellovibrio invasion and a zonal mode of predator-elongation. This process is followed by unconventional, multi-point and synchronous septation of the intracellular Bdellovibrio, accommodating odd- and even-numbered progeny formation by non-binary division.
Citation
Kuru, E., Lambert, C., Jonathon, R., Till, R., Ducret, A., Derouaux, A., Gray, J., Biboy, J., Vollmer, W., VanNieuwenhze, M., Brun, Y., & Sockett, L. (2017). Fluorescent D-amino-acids reveal bi-cellular cell wall modifications important for Bdellovibrio bacteriovorus predation. Nature Microbiology, 2(12), 1648-1657. https://doi.org/10.1038/s41564-017-0029-y
Journal Article Type | Article |
---|---|
Acceptance Date | Aug 11, 2017 |
Online Publication Date | Oct 3, 2017 |
Publication Date | 2017-12 |
Deposit Date | Sep 7, 2017 |
Publicly Available Date | Oct 12, 2017 |
Journal | Nature Microbiology |
Electronic ISSN | 2058-5276 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 2 |
Issue | 12 |
Pages | 1648-1657 |
DOI | https://doi.org/10.1038/s41564-017-0029-y |
Public URL | https://nottingham-repository.worktribe.com/output/885977 |
Publisher URL | https://www.nature.com/articles/s41564-017-0029-y |
Contract Date | Sep 7, 2017 |
Files
FDAA Bdello-Nature microbiology Final with figs.pdf
(16.9 Mb)
PDF
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