Evaluation of combinations of putative anti-biofilm agents and antibiotics to eradicate biofilms of Staphylococcus aureus and Pseudomonas aeruginosa

Belfield, Katherine; Bayston, Roger; Hajduk, Nadzieja; Levell, Georgia; Birchall, John P.; Daniel, Matija

Abstract

Objectives

To evaluate potential anti-biofilm agents for their ability to enhance the activity of antibiotics for local treatment of localized biofilm infections.
Methods

Staphylococcus aureus and Pseudomonas aeruginosa in vitro biofilm models were developed. The putative antibiotic enhancers N-acetylcysteine, acetylsalicylic acid, sodium salicylate, recombinant human deoxyribonuclease I, dispersin B, hydrogen peroxide and Johnson’s Baby Shampoo (JBS) were tested for their anti-biofilm activity alone and their ability to enhance the activity of antibiotics for 7 or 14 days, against 5 day old biofilms. The antibiotic enhancers were paired with rifampicin and clindamycin against S. aureus and gentamicin and ciprofloxacin against P. aeruginosa. Isolates from biofilms that were not eradicated were tested for antibiotic resistance.
Results

Antibiotic levels 10× MIC and 100× MIC significantly reduced biofilm, but did not consistently eradicate it. Antibiotics at 100× MIC with 10% JBS for 14 days was the only treatment to eradicate both staphylococcal and pseudomonal biofilms. Recombinant human deoxyribonuclease I significantly reduced staphylococcal biofilm. Emergence of resistance of surviving isolates was minimal and was often associated with the small colony variant phenotype.
Conclusions

JBS enhanced the activity of antibiotics and several other promising anti-biofilm agents were identified. Antibiotics with 10% JBS eradicated biofilms produced by both organisms. Such combinations might be useful in local treatment of localized biofilm infections.

Citation

Belfield, K., Bayston, R., Hajduk, N., Levell, G., Birchall, J. P., & Daniel, M. (2017). Evaluation of combinations of putative anti-biofilm agents and antibiotics to eradicate biofilms of Staphylococcus aureus and Pseudomonas aeruginosa. Journal of Antimicrobial Chemotherapy, 72(9), 2531-2538. https://doi.org/10.1093/jac/dkx192

• Journal Article Type: Article
• Acceptance Date: May 23, 2017
• Online Publication Date: Jul 4, 2017
• Publication Date: Sep 1, 2017
• Deposit Date: Aug 22, 2017
• Publicly Available Date: Aug 22, 2017
• Journal: Journal of Antimicrobial Chemotherapy
• Print ISSN: 0305-7453
• Electronic ISSN: 1460-2091
• Publisher: Oxford University Press
• Peer Reviewed: Peer Reviewed
• Volume: 72
• Issue: 9
• Pages: 2531-2538
• DOI: https://doi.org/10.1093/jac/dkx192
• Public URL: https://nottingham-repository.worktribe.com/output/879796
• Publisher URL: https://doi.org/10.1093/jac/dkx192
• Additional Information: This is a pre-copyedited, author-produced version of an article accepted for publication in Journal of Antimicrobial Chemotherapy following peer review. The version of record Katherine Belfield, Roger Bayston, Nadzieja Hajduk, Georgia Levell, John P. Birchall, Matija Daniel; Evaluation of combinations of putative anti-biofilm agents and antibiotics to eradicate biofilms of Staphylococcus aureus and Pseudomonas aeruginosa, Journal of Antimicrobial Chemotherapy, Volume 72, Issue 9, 1 September 2017, Pages 2531–2538 is available online at: https://doi.org/10.1093/jac/dkx192.