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Ki67 expression in invasive breast cancer: the use of tissue microarrays compared with whole tissue sections

Muftah, Abir A.; Aleskandarany, Mohammed A.; Al-kaabi, Methaq M.; Sonbul, Sultan N.; Diez-Rodriguez, Maria; Nolan, Christopher C.; Caldas, Carlos; Ellis, Ian O.; Rakha, Emad A.; Green, Andrew R.

Authors

Abir A. Muftah

Mohammed A. Aleskandarany

Methaq M. Al-kaabi

Sultan N. Sonbul

Maria Diez-Rodriguez

Christopher C. Nolan

Carlos Caldas

Emad A. Rakha

Andrew R. Green



Abstract

Background: Although the prognostic value of Ki67 in breast cancer is well documented, using optimal cut-points for patient stratification, reproducibility of the scoring and interpretation of the results remain a matter of debate particularly when using Tissue Microarrays (TMAs). This study aims to assess Ki67 expression assessed on TMAs and their matched whole tissue sections (WTS). Moreover, whether the cut-off used for WTS is reproducible on TMA in BC molecular classes and the association between Ki67 expression cut-off, assessed on TMAs and WTS, and clinicopathological parameters and patient outcome will also be tested.
Method: A large series (n=707) of primary invasive breast tumours were immunostained for Ki67 using both TMA and WTS and assessed as percentage staining and correlated with each other, clinicopathological parameters and patient outcome. In addition, MKI67 mRNA expression was correlated with Ki67 protein levels on WTS and TMAs in a subset of cases included in the METABRIC study.
Results: There was moderate concordance in Ki67 expression between WTS and TMA when analysed as a continuous variable (Intraclass correlation coefficient=0.61) and low concordance when dichotomised (kappa-value=0.3). TMA showed low levels of Ki67 with mean percentage of expression 35% 22% on WTS and TMA, respectively. MKI67 mRNA expression was significantly correlated with protein expression determined on WTS (Spearman Correlation, r=0.52) and to a lesser extent on TMA (r=0.34) (p

Journal Article Type Article
Publication Date 2017-07
Journal Breast Cancer Research and Treatment
Print ISSN 0167-6806
Electronic ISSN 1573-7217
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 164
Issue 2
Pages 341-348
APA6 Citation Muftah, A. A., Aleskandarany, M. A., Al-kaabi, M. M., Sonbul, S. N., Diez-Rodriguez, M., Nolan, C. C., …Green, A. R. (2017). Ki67 expression in invasive breast cancer: the use of tissue microarrays compared with whole tissue sections. Breast Cancer Research and Treatment, 164(2), 341-348. https://doi.org/10.1007/s10549-017-4270-0
DOI https://doi.org/10.1007/s10549-017-4270-0
Keywords Ki67, Breast Cancer, Immunohistochemistry, Prognosis, METABRIC
Publisher URL https://link.springer.com/article/10.1007%2Fs10549-017-4270-0
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information The final publication is available at Springer via http://dx.doi.org/10.1007/s10549-017-4270-0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0







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