Skip to main content

Research Repository

See what's under the surface

Advanced Search

Differential gene expression at different stages of mesocarp development in high- and low-yielding oil palm

Wong, Yick Ching; Teh, Huey Fang; Mebus, Katharina; Ooi, Tony Eng Keong; Kwong, Qi Bin; Koo, Ka Loo; Ong, Chuang Kee; Mayes, Sean; Chew, Fook Tim; Appleton, David R.; Kulaveerasingam, Harikrishna

Authors

Yick Ching Wong

Huey Fang Teh

Katharina Mebus

Tony Eng Keong Ooi

Qi Bin Kwong

Ka Loo Koo

Chuang Kee Ong

Sean Mayes

Fook Tim Chew

David R. Appleton

Harikrishna Kulaveerasingam



Abstract

Background

The oil yield trait of oil palm is expected to involve multiple genes, environmental influences and interactions. Many of the underlying mechanisms that contribute to oil yield are still poorly understood. In this study, we used a microarray approach to study the gene expression profiles of mesocarp tissue at different developmental stages, comparing genetically related high- and low- oil yielding palms to identify genes that contributed to the higher oil-yielding palm and might contribute to the wider genetic improvement of oil palm breeding populations.
Results

A total of 3412 (2001 annotated) gene candidates were found to be significantly differentially expressed between high- and low-yielding palms at at least one of the different stages of mesocarp development evaluated. Gene Ontologies (GO) enrichment analysis identified 28 significantly enriched GO terms, including regulation of transcription, fatty acid biosynthesis and metabolic processes. These differentially expressed genes comprise several transcription factors, such as, bHLH, Dof zinc finger proteins and MADS box proteins. Several genes involved in glycolysis, TCA, and fatty acid biosynthesis pathways were also found up-regulated in high-yielding oil palm, among them; pyruvate dehydrogenase E1 component Subunit Beta (PDH), ATP-citrate lyase, β- ketoacyl-ACP synthases I (KAS I), β- ketoacyl-ACP synthases III (KAS III) and ketoacyl-ACP reductase (KAR). Sucrose metabolism-related genes such as Invertase, Sucrose Synthase 2 and Sucrose Phosphatase 2 were found to be down-regulated in high-yielding oil palms, compared to the lower yield palms.
Conclusions

Our findings indicate that a higher carbon flux (channeled through down-regulation of the Sucrose Synthase 2 pathway) was being utilized by up-regulated genes involved in glycolysis, TCA and fatty acid biosynthesis leading to enhanced oil production in the high-yielding oil palm. These findings are an important stepping stone to understand the processes that lead to production of high-yielding oil palms and have implications for breeding to maximize oil production.

Journal Article Type Article
Publication Date Jun 21, 2017
Journal BMC Genomics
Electronic ISSN 1471-2164
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 18
Issue 1
Article Number 470
APA6 Citation Wong, Y. C., Teh, H. F., Mebus, K., Ooi, T. E. K., Kwong, Q. B., Koo, K. L., …Kulaveerasingam, H. (2017). Differential gene expression at different stages of mesocarp development in high- and low-yielding oil palm. BMC Genomics, 18(1), doi:10.1186/s12864-017-3855-7
DOI https://doi.org/10.1186/s12864-017-3855-7
Keywords Oil palm; Elaeis guineensis; Gene expression; Expression microarray; Oil yield
Publisher URL https://bmcgenomics.biomedcentral.com/articles/10.1186/s12864-017-3855-7
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

Files

Wong_et_al-2017-BMC_Genomics.pdf (1.7 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





You might also like



Downloadable Citations

;