Fernanda de Oliveira Barbosa
Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken
Barbosa, Fernanda de Oliveira; Freitas Neto, Oliveiro Caetano de; Batista, Diego Felipe Alves; Almeida, Adriana Maria de; Rubio, Marcela da Silva; Alves, Lucas Bocchini Rodrigues; Vasconcelos, Rosemeire de Oliveira; Barrow, Paul Andrew; Berchieri Junior, Angelo
Authors
Oliveiro Caetano de Freitas Neto
Diego Felipe Alves Batista
Adriana Maria de Almeida
Marcela da Silva Rubio
Lucas Bocchini Rodrigues Alves
Rosemeire de Oliveira Vasconcelos
Paul Andrew Barrow
Angelo Berchieri Junior
Abstract
Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken.
Citation
Barbosa, F. D. O., Freitas Neto, O. C. D., Batista, D. F. A., Almeida, A. M. D., Rubio, M. D. S., Alves, L. B. R., …Berchieri Junior, A. (in press). Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken. Brazilian Journal of Microbiology, https://doi.org/10.1016/j.bjm.2017.01.012
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 20, 2017 |
Online Publication Date | Jun 7, 2017 |
Deposit Date | Aug 23, 2017 |
Publicly Available Date | Aug 23, 2017 |
Journal | Brazilian Journal of Microbiology |
Electronic ISSN | 1517-8382 |
Peer Reviewed | Peer Reviewed |
DOI | https://doi.org/10.1016/j.bjm.2017.01.012 |
Keywords | Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation |
Public URL | https://nottingham-repository.worktribe.com/output/864522 |
Publisher URL | http://www.sciencedirect.com/science/article/pii/S1517838216307080?via%3Dihub |
Contract Date | Aug 23, 2017 |
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