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Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken

Barbosa, Fernanda de Oliveira; Freitas Neto, Oliveiro Caetano de; Batista, Diego Felipe Alves; Almeida, Adriana Maria de; Rubio, Marcela da Silva; Alves, Lucas Bocchini Rodrigues; Vasconcelos, Rosemeire de Oliveira; Barrow, Paul Andrew; Berchieri Junior, Angelo

Authors

Fernanda de Oliveira Barbosa

Oliveiro Caetano de Freitas Neto

Diego Felipe Alves Batista

Adriana Maria de Almeida

Marcela da Silva Rubio

Lucas Bocchini Rodrigues Alves

Rosemeire de Oliveira Vasconcelos

Paul Andrew Barrow

Angelo Berchieri Junior



Abstract

Salmonella Enteritidis causes fowl paratyphoid in poultry and is frequently associated to outbreaks of food-borne diseases in humans. The role of flagella and flagella-mediated motility into host-pathogen interplay is not fully understood and requires further investigation. In this study, one-day-old chickens were challenged orally with a wild-type strain Salmonella Enteritidis, a non-motile but fully flagellated (SE ΔmotB) or non-flagellated (SE ΔfliC) strain to evaluate their ability to colonise the intestine and spread systemically and also of eliciting gross and histopathological changes. SE ΔmotB and SE ΔfliC were recovered in significantly lower numbers from caecal contents in comparison with Salmonella Enteritidis at early stages of infection (3 and 5 dpi). The SE ΔmotB strain, which synthesises paralysed flagella, showed poorer intestinal colonisation ability than the non-flagellated SE ΔfliC. Histopathological analyses demonstrated that the flagellated strains induced more intense lymphoid reactivity in liver, ileum and caeca. Thus, in the present study the flagellar structure and motility seemed to play a role in the early stages of the intestinal colonisation by Salmonella Enteritidis in the chicken.

Citation

Barbosa, F. D. O., Freitas Neto, O. C. D., Batista, D. F. A., Almeida, A. M. D., Rubio, M. D. S., Alves, L. B. R., …Berchieri Junior, A. (in press). Contribution of flagella and motility to gut colonisation and pathogenicity of Salmonella Enteritidis in the chicken. Brazilian Journal of Microbiology, https://doi.org/10.1016/j.bjm.2017.01.012

Journal Article Type Article
Acceptance Date Jan 20, 2017
Online Publication Date Jun 7, 2017
Deposit Date Aug 23, 2017
Publicly Available Date Aug 23, 2017
Journal Brazilian Journal of Microbiology
Electronic ISSN 1517-8382
Peer Reviewed Peer Reviewed
DOI https://doi.org/10.1016/j.bjm.2017.01.012
Keywords Mutation ; Flagellum ; Virulence factors ; Poultry ; Gut colonisation
Public URL http://eprints.nottingham.ac.uk/id/eprint/45100
Publisher URL http://www.sciencedirect.com/science/article/pii/S1517838216307080?via%3Dihub
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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