Muscle carnitine availability plays a central role in regulating fuel metabolism in the rodent

Porter, Craig; Constantin-Teodosiu, Dumitru; Constantin, Despina; Leighton, Brendan; Poucher, Simon M.; Greenhaff, Paul L.

Abstract

The body carnitine pool is primarily confined to skeletal muscle, where it regulates carbohydrate (CHO) and fat usage. Mildronate (3-(2,2,2-trimethylhydrazinium)-propionate) inhibits carnitine synthesis and tissue uptake, but the impact of carnitine depletion on whole-body fuel selection, muscle fuel metabolism and its molecular regulation is under-investigated. Male lean Zucker rats received water (control, n=8) or mildronate-supplemented water (mildronate, n=8) for 10 days (1.6 g.kg-1 body mass (bm).day-1 day 1-2, 0.8 g.kg-1 bm.day-1 thereafter). From day 7-10, animals were housed in indirect calorimetry chambers after which soleus muscle and liver were harvested. Food and fluid intake, weight gain and physical activity levels were similar between groups from day 7-10. Compared to control, mildronate depleted muscle total carnitine (P

Citation

Porter, C., Constantin-Teodosiu, D., Constantin, D., Leighton, B., Poucher, S. M., & Greenhaff, P. L. (2017). Muscle carnitine availability plays a central role in regulating fuel metabolism in the rodent. Journal of Physiology, 595(17), 5765-5780. https://doi.org/10.1113/JP274415

• Journal Article Type: Article
• Acceptance Date: May 31, 2017
• Online Publication Date: Jun 12, 2017
• Publication Date: Sep 1, 2017
• Deposit Date: Jul 3, 2017
• Publicly Available Date: Jun 13, 2018
• Journal: Journal of Physiology
• Print ISSN: 0022-3751
• Electronic ISSN: 1469-7793
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 595
• Issue: 17
• Pages: 5765-5780
• DOI: https://doi.org/10.1113/JP274415
• Keywords: Carnitine, Muscle fuel selection, Fat and carbohydrate metabolism
• Public URL: https://nottingham-repository.worktribe.com/output/863867
• Publisher URL: https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/JP274415
• Additional Information: This is the peer reviewed version of the article, which has been published in final form at https://physoc.onlinelibrary.wiley.com/doi/full/10.1113/JP274415. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving.