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Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis

Grewal, Surbhi; Gardiner, Laura-Jayne; Ndreca, Barobora; Knight, Emilie; Moore, Graham; King, Ian P.; King, Julie

Authors

Surbhi Grewal surbhi.grewal@nottingham.ac.uk

Laura-Jayne Gardiner Laura-Jayne.Gardiner@earlham.ac.uk

Barobora Ndreca barbora.ndreca@nottingham.ac.uk

Emilie Knight emilie.knight@jic.ac.uk

Graham Moore graham.moore@jic.ac.uk

Ian P. King ian.king@nottingham.ac.uk

Julie King sbzjk@exmail.nottingham.ac.uk



Abstract

Gametocidal (Gc) chromosomes or elements in species such as Aegilops sharonensis are preferentially transmitted to the next generation through both the male and female gametes when introduced into wheat. Furthermore, any genes, e.g. genes that control agronomically important traits, showing complete linkage with gametocidal elements, are also transmitted preferentially to the next generation without the need for selection. The mechanism for the preferential transmission of the gametocidal elements appears to occur by the induction of extensive chromosome damage in any gametes that lack the gametocidal chromosome in question. Previous studies on the mechanism of the gametocidal action in Ae. sharonensis indicates that at least two-linked elements are involved. The first, the ‘breaker’ element, induces chromosome breakage in gametes, which have lost the gametocidal elements while the second, the ‘inhibitor’ element, prevents the chromosome breakage action of the ‘breaker’ element in gametes, which carry the Gc elements. In this study, we have used comparative genomic studies to map 54 single nucleotide polymorphism (SNP) markers in an Ae. sharonensis 4SshL introgression segment in wheat and have also identified 18 candidate genes in Ae. sharonensis for the ‘breaker’ element through targeted sequencing of this 4SshL introgression segment. This valuable genomic resource will aide in further mapping the Gc locus that could be exploited in wheat breeding to produce new, superior varieties of wheat.

Journal Article Type Article
Publication Date May 11, 2017
Journal Plant Genome
Electronic ISSN 1940-3372
Publisher Crop Science Society of America
Peer Reviewed Peer Reviewed
Volume 10
Issue 2
APA6 Citation Grewal, S., Gardiner, L., Ndreca, B., Knight, E., Moore, G., King, I. P., & King, J. (2017). Comparative mapping and targeted-capture sequencing of the gametocidal loci in Aegilops sharonensis. Plant Genome, The, 10(2), https://doi.org/10.3835/plantgenome2016.09.0090
DOI https://doi.org/10.3835/plantgenome2016.09.0090
Publisher URL https://dl.sciencesocieties.org/publications/tpg/abstracts/10/2/plantgenome2016.09.0090
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0


tpg-10-2-plantgenome2016.09.0090.pdf (2.7 Mb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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