Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells

Xu, Sha-Sha; Liu, Qing-Mei; Xiao, An-Feng; Maleki, Soheila J.; Alcocer, Marcos; Gao, Yuan-Yuan; Cao, Min-Jie; Liu, Guang-Ming

Abstract

In the present study, the anti-food allergy activity of Eucheuma cottonii sulfated oligosaccharide (ESO) was investigated. ESO was obtained by enzymatic degradation and purified by column chromatography. RBL-2H3 cells and BALB/c mouse model were used to test the anti-food allergy activity of ESO. The effects of ESO on the regulatory T (Treg) cells and bone marrow-derived mast cells (BMMCs) were investigated by flow cytometry. The results of in vivo assay showed that ESO decreased the levels of mast cell protease-1 and histamine and inhibited the levels of specific IgE by 77.7%. In addition, the production of interleukin (IL)-4 and IL-13 was diminished in the ESO groups compared to the non-ESO-treated group. Furthermore, ESO could up-regulate Treg cells by 22.2−97.1%. In conclusion, ESO decreased the allergy response in mice by reducing basophil degranulation, up-regulating Treg cells via Forkhead box protein 3 (Foxp3), and releasing IL-10. ESO may have preventive and therapeutic potential in allergic disease.

Citation

Xu, S.-S., Liu, Q.-M., Xiao, A.-F., Maleki, S. J., Alcocer, M., Gao, Y.-Y., …Liu, G.-M. (2017). Eucheuma cottonii sulfated oligosaccharides decrease food allergic responses in animal models by up-regulating regulatory T (Treg) cells. Journal of Agricultural and Food Chemistry, 65(15), https://doi.org/10.1021/acs.jafc.7b00389

• Journal Article Type: Article
• Acceptance Date: Mar 30, 2017
• Publication Date: Mar 30, 2017
• Deposit Date: Sep 6, 2017
• Journal: Journal of Agricultral and Food Chemistry
• Print ISSN: 0021-8561
• Electronic ISSN: 1520-5118
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 65
• Issue: 15
• DOI: https://doi.org/10.1021/acs.jafc.7b00389
• Keywords: Eucheuma cottonii sulfated oligosaccharide (ESO); food allergy; mast cells; murine model; Treg cells
• Public URL: https://nottingham-repository.worktribe.com/output/853470
• Publisher URL: http://pubs.acs.org/doi/abs/10.1021/acs.jafc.7b00389
• Contract Date: Sep 6, 2017