�lex Bay�s
Evolution of complexity in the zebrafish synapse proteome
Bay�s, �lex; Bayes, Alex; Collins, Mark O.; Reig-Vader, Rrita; Gou, Gemma; Goulding, David; Izquierdo, Abril; Choudhary, Jyoti S.; Emes, Richard D.; Grant, Seth G.N.
Authors
Alex Bayes
Mark O. Collins
Rrita Reig-Vader
Gemma Gou
David Goulding
Abril Izquierdo
Jyoti S. Choudhary
Richard D. Emes
Seth G.N. Grant
Abstract
The proteome of human brain synapses is highly complex and mutated in over 130 diseases. This complexity arose from two whole genome duplications early in the vertebrate lineage. Zebrafish are used in modelling human diseases, however its synapse proteome is uncharacterised and whether the teleost-specific genome duplication (TSGD) influenced complexity is unknown. We report the characterisation of the proteomes and ultrastructure of central synapses in zebrafish and analyse the importance of the TSGD. While the TSGD increases overall synapse proteome complexity, the Post Synaptic Density (PSD) proteome of zebrafish has lower complexity than mammals. A highly conserved set of ~1000 proteins is shared across vertebrates. PSD ultrastructural features are also conserved. Lineage-specific proteome differences indicate vertebrate species evolved distinct synapse types and functions. The datasets are a resource for a wide range of studies and have important implications for the use of zebrafish in modelling human synaptic diseases.
Citation
Bayés, À., Bayes, A., Collins, M. O., Reig-Vader, R., Gou, G., Goulding, D., …Grant, S. G. (in press). Evolution of complexity in the zebrafish synapse proteome. Nature Communications, 8, Article 14613. https://doi.org/10.1038/ncomms14613
Journal Article Type | Article |
---|---|
Acceptance Date | Jan 15, 2017 |
Online Publication Date | Mar 2, 2017 |
Deposit Date | Mar 3, 2017 |
Publicly Available Date | Mar 3, 2017 |
Journal | Nature Communications |
Electronic ISSN | 2041-1723 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 8 |
Article Number | 14613 |
DOI | https://doi.org/10.1038/ncomms14613 |
Public URL | https://nottingham-repository.worktribe.com/output/848393 |
Publisher URL | http://www.nature.com/articles/ncomms14613 |
Contract Date | Mar 3, 2017 |
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Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
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