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Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches

Baki, Abdulrahman; Rahman, Cheryl V.; White, Lisa J.; Scurr, David J.; Qutachi, Omar; Shakesheff, Kevin M.

Authors

Abdulrahman Baki

Cheryl V. Rahman

Lisa J. White

David J. Scurr

Omar Qutachi



Abstract

Injectable poly (dl-lactic-co-glycolic acid) (PdlLGA) microspheres are promising candidates as biodegradable controlled release carriers for drug and cell delivery applications; however, they have limited functional groups on the surface to enable dense grafting of tissue specific biocompatible molecules. In this study we have evaluated surface adsorption, entrapment and oxygen plasma treatment as three approaches to modify the surfaces of PdlLGA microspheres with gelatine methacrylate (gel-MA) as a biocompatible and photo cross-linkable macromolecule. Time of flight secondary ion mass spectroscopy (TOF SIMS) and X-ray photoelectron spectroscopy (XPS) were used to detect and quantify gel-MA on the surfaces. Fluorescent and scanning electron microscopies (SEM) were used to image the topographical changes. Human mesenchymal stem cells (hMSCs) of immortalised cell line were cultured on the surface of gel-MA modified PdlLGA microspheres and Presto-Blue assay was used to study the effect of different surface modifications on cell proliferation. Data analysis showed that the oxygen plasma treatment approach resulted in the highest density of gel-MA deposition. This study supports oxygen plasma treatment as a facile approach to modify the surface of injectable PdlLGA microspheres with macromolecules such as gel-MA to enhance proliferation rate of injected cells and potentially enable further grafting of tissue specific molecules.

Journal Article Type Article
Publication Date 2017-04
Journal Acta Biomaterialia
Print ISSN 1742-7061
Electronic ISSN 1878-7568
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 53
Pages 450-459
APA6 Citation Baki, A., Rahman, C. V., White, L. J., Scurr, D. J., Qutachi, O., & Shakesheff, K. M. (2017). Surface modification of PdlLGA microspheres with gelatine methacrylate: evaluation of adsorption, entrapment, and oxygen plasma treatment approaches. Acta Biomaterialia, 53, 450-459. https://doi.org/10.1016/j.actbio.2017.01.042
DOI https://doi.org/10.1016/j.actbio.2017.01.042
Keywords Surface modification ; PDLLGA microspheres ; Gelatine methacrylate ; Drug delivery ; Tissue engineering
Publisher URL https://doi.org/10.1016/j.actbio.2017.01.042
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information This article is maintained by: Elsevier; Article Title: Surface modification of PdlLGA microspheres with gelatine methacrylate: Evaluation of adsorption, entrapment, and oxygen plasma treatment approaches; Journal Title: Acta Biomaterialia; CrossRef DOI link to publisher maintained version: https://doi.org/10.1016/j.actbio.2017.01.042; Content Type: article; Copyright: © 2017 Acta Materialia Inc. Published by Elsevier Ltd.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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