Mustafa A. Karwad
Oleoylethanolamine and palmitoylethanolamine modulate intestinal permeability in vitro via TRPV1 and PPARα
Karwad, Mustafa A.; Macpherson, Tara; Wang, Bo; Theophilidou, Elena; Sarmad, Sarir; Barrett, David A.; Larvin, Michael; Wright, Karen L.; Lund, Jonathan N.; O'Sullivan, Saoirse
Authors
Tara Macpherson
Bo Wang
Elena Theophilidou
Dr SARIR SARMAD Sarir.Sarmad@nottingham.ac.uk
Senior Teaching Technician
David A. Barrett
Michael Larvin
Karen L. Wright
JONATHAN LUND JON.LUND@NOTTINGHAM.AC.UK
Clinical Associate Professor
Saoirse O'Sullivan
Abstract
Cannabinoids modulate intestinal permeability through cannabinoid receptor 1 (CB1). The endocannabinoid-like compounds oleoylethanolamine (OEA) and palmitoylethanolamine (PEA) play an important role in digestive regulation, and we hypothesized they would also modulate intestinal permeability. Transepithelial electrical resistance (TEER) was measured in human Caco-2 cells to assess permeability after application of OEA and PEA and relevant antagonists. Cells treated with OEA and PEA were stained for cytoskeletal F-actin changes and lysed for immunoassay. OEA and PEA were measured by liquid chromatography–tandem mass spectrometry. OEA (applied apically, logEC50 −5.4) and PEA (basolaterally, logEC50 −4.9; apically logEC50 −5.3) increased Caco-2 resistance by 20–30% via transient receptor potential vanilloid (TRPV)-1 and peroxisome proliferator-activated receptor (PPAR)-α. Preventing their degradation (by inhibiting fatty acid amide hydrolase) enhanced the effects of OEA and PEA. OEA and PEA induced cytoskeletal changes and activated focal adhesion kinase and ERKs 1/2, and decreased Src kinases and aquaporins 3 and 4. In Caco-2 cells treated with IFNγ and TNFα, OEA (via TRPV1) and PEA (via PPARα) prevented or reversed the cytokine-induced increased permeability compared to vehicle (0.1% ethanol). PEA (basolateral) also reversed increased permeability when added 48 or 72 h after cytokines (P < 0.001, via PPARα). Cellular and secreted levels of OEA and PEA (P < 0.001–0.001) were increased in response to inflammatory mediators. OEA and PEA have endogenous roles and potential therapeutic applications in conditions of intestinal hyperpermeability and inflammation.
Citation
Karwad, M. A., Macpherson, T., Wang, B., Theophilidou, E., Sarmad, S., Barrett, D. A., …O'Sullivan, S. (2017). Oleoylethanolamine and palmitoylethanolamine modulate intestinal permeability in vitro via TRPV1 and PPARα. FASEB Journal, 31(2), 469-481. https://doi.org/10.1096/fj.201500132
Journal Article Type | Article |
---|---|
Acceptance Date | Sep 1, 2016 |
Online Publication Date | Sep 13, 2016 |
Publication Date | 2017-02 |
Deposit Date | Aug 22, 2017 |
Publicly Available Date | Aug 22, 2017 |
Journal | FASEB Journal |
Print ISSN | 0892-6638 |
Electronic ISSN | 1530-6860 |
Publisher | Federation of American Society of Experimental Biology (FASEB) |
Peer Reviewed | Peer Reviewed |
Volume | 31 |
Issue | 2 |
Pages | 469-481 |
DOI | https://doi.org/10.1096/fj.201500132 |
Keywords | gut, cannibinoid, nuclear receptor |
Public URL | https://nottingham-repository.worktribe.com/output/818113 |
Publisher URL | http://www.fasebj.org/content/31/2/469 |
Contract Date | Aug 22, 2017 |
Files
accepted FASEB paper.pdf
(1.6 Mb)
PDF
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