Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging

Sinjab, Faris; Kong, Kenny; Gibson, Graham; Varma, Sandeep; Williams, Hywel C.; Padgett, Miles; Notingher, Ioan

Abstract

We describe a multifocal Raman micro-spectroscopy detection method based on a digital micromirror device, which allows for simultaneous “power-sharing” acquisition of Raman spectra from ad hoc sampling points. As the locations of the points can be rapidly updated in real-time via software control of a liquid-crystal spatial light modulator (LC-SLM), this technique is compatible with automated adaptive- and selective-sampling Raman spectroscopy techniques, the latter of which has previously been demonstrated for fast diagnosis of skin cancer tissue resections. We describe the performance of this instrument and show examples of multiplexed measurements on a range of test samples. Following this, we show the feasibility of reducing measurement time for power-shared multifocal Raman measurements combined with confocal auto-fluorescence imaging to provide guided diagnosis of tumours in human skin samples.

Citation

Sinjab, F., Kong, K., Gibson, G., Varma, S., Williams, H. C., Padgett, M., & Notingher, I. (2016). Tissue diagnosis using power-sharing multifocal Raman micro-spectroscopy and auto-fluorescence imaging. Biomedical Optics Express, 7(8), 2993–3006. https://doi.org/10.1364/BOE.7.002993

• Journal Article Type: Article
• Acceptance Date: Jun 6, 2016
• Online Publication Date: Jul 11, 2016
• Publication Date: Aug 1, 2016
• Deposit Date: Jul 18, 2016
• Publicly Available Date: Jul 18, 2016
• Journal: Biomedical Optics Express
• Electronic ISSN: 2156-7085
• Publisher: Optical Society of America
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 8
• Pages: 2993–3006
• DOI: https://doi.org/10.1364/BOE.7.002993
• Public URL: https://nottingham-repository.worktribe.com/output/796467
• Publisher URL: https://www.osapublishing.org/DirectPDFAccess/E1D19248-E17F-272F-4B783ED8BD54B1F4_345500/boe-7-8-2993.pdf?da=1&id=345500&seq=0&mobile=no
• Contract Date: Jul 18, 2016