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Optimising performance of a confocal fluorescence microscope with a differential pinhole

Kakade, Rohan; Walker, John G.; Phillips, Andrew J.

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Authors

Rohan Kakade

John G. Walker

Andrew J. Phillips



Abstract

The signal-to-noise ratio (SNR)-resolution trade-off is of great importance to bio-imaging applications where the aim is to image the sample using as little light as possible without significantly sacrificing image quality. In this paper the inherent SNR-resolution tradeoff in Confocal Fluorescence Microscopy (CFM) systems is presented by means of an effective tradeoff curve. A CFM system that employs a differential pinhole detection scheme has recently been shown to offer increased resolution, but at the expense of SNR. An optimum profile for the differential pinhole is identified in this paper that offers improved performance over a conventional (circular pinhole) system. The performance enhancement is illustrated through computer simulation.

Citation

Kakade, R., Walker, J. G., & Phillips, A. J. (2015). Optimising performance of a confocal fluorescence microscope with a differential pinhole. Measurement Science and Technology, 27(1), https://doi.org/10.1088/0957-0233/27/1/015401

Journal Article Type Article
Acceptance Date Nov 6, 2015
Publication Date Nov 27, 2015
Deposit Date Jul 28, 2016
Publicly Available Date Jul 28, 2016
Journal Measurement Science and Technology
Print ISSN 0957-0233
Electronic ISSN 1361-6501
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 27
Issue 1
DOI https://doi.org/10.1088/0957-0233/27/1/015401
Keywords confocal fluorescence microscopy, resolution improvement, differential pinhole, image detection
Public URL https://nottingham-repository.worktribe.com/output/765371
Publisher URL http://iopscience.iop.org/article/10.1088/0957-0233/27/1/015401/meta
Contract Date Jul 28, 2016

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