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Optimising performance of a confocal fluorescence microscope with a differential pinhole

Kakade, Rohan; Walker, John G.; Phillips, Andrew J.

Authors

Rohan Kakade

John G. Walker john.walker@nottingham.ac.uk

Andrew J. Phillips andrew.phillips@nottingham.ac.uk



Abstract

The signal-to-noise ratio (SNR)-resolution trade-off is of great importance to bio-imaging applications where the aim is to image the sample using as little light as possible without significantly sacrificing image quality. In this paper the inherent SNR-resolution tradeoff in Confocal Fluorescence Microscopy (CFM) systems is presented by means of an effective tradeoff curve. A CFM system that employs a differential pinhole detection scheme has recently been shown to offer increased resolution, but at the expense of SNR. An optimum profile for the differential pinhole is identified in this paper that offers improved performance over a conventional (circular pinhole) system. The performance enhancement is illustrated through computer simulation.

Journal Article Type Article
Publication Date Nov 27, 2015
Journal Measurement Science and Technology
Print ISSN 0957-0233
Electronic ISSN 1361-6501
Publisher IOP Publishing
Peer Reviewed Peer Reviewed
Volume 27
Issue 1
APA6 Citation Kakade, R., Walker, J. G., & Phillips, A. J. (2015). Optimising performance of a confocal fluorescence microscope with a differential pinhole. Measurement Science and Technology, 27(1), https://doi.org/10.1088/0957-0233/27/1/015401
DOI https://doi.org/10.1088/0957-0233/27/1/015401
Keywords confocal fluorescence microscopy, resolution improvement, differential pinhole, image detection
Publisher URL http://iopscience.iop.org/article/10.1088/0957-0233/27/1/015401/meta
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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