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Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity

Marti, E.; Wang, X.; Jambari, N.N.; Rhyner, C.; Olzhausen, J.; Pérez-Barea, J.J.; Figueredo, Grazziela P.; Alcocer, Marcos J.C.

Authors

E. Marti

X. Wang

N.N. Jambari

C. Rhyner

J. Olzhausen

J.J. Pérez-Barea

Grazziela P. Figueredo gzf@cs.nott.ac.uk

Marcos J.C. Alcocer



Abstract

Insect bite hypersensitivity (IBH) is a seasonal recurrent skin allergy of horses caused by IgE-mediated reactions to allergens present in the saliva of biting insects of the genus Culicoides, and possibly also Simulium and Stomoxys species. In this work we show that protein microarrays containing complex extracts and pure proteins, including recombinant Culicoides allergens, can be used as a powerful technique for the diagnosis of IBH. Besides the obvious advantages such as general profiling and use of few microliters of samples, this microarray technique permits automation and allows the generation of mathematical models with the calculation of individual risk profiles that can support the clinical diagnosis of allergic diseases. After selection of variables on influence on the projection (VIP), the observed values of sensitivity and specificity were 1.0 and 0.967, respectively. This confirms the highly discriminatory power of this approach for IBH and made it possible to attain a robust predictive mathematical model for this disease. It also further demonstrates the specificity of the protein array method on identifying a particular IgE-mediated disease when the sensitising allergen group is known.

Journal Article Type Article
Publication Date Oct 15, 2015
Journal Veterinary Immunology and Immunopathology
Print ISSN 0165-2427
Electronic ISSN 1873-2534
Publisher Elsevier
Peer Reviewed Peer Reviewed
Volume 167
Issue 3-4
APA6 Citation Marti, E., Wang, X., Jambari, N., Rhyner, C., Olzhausen, J., Pérez-Barea, J., …Alcocer, M. J. (2015). Novel in vitro diagnosis of equine allergies using a protein array and mathematical modelling approach: a proof of concept using insect bite hypersensitivity. Veterinary Immunology and Immunopathology, 167(3-4), doi:10.1016/j.vetimm.2015.06.013
DOI https://doi.org/10.1016/j.vetimm.2015.06.013
Keywords Protein array; Allergens; Chemometrics; IgE; Horse
Publisher URL https://www.sciencedirect.com/science/article/pii/S0165242715001397?via%3Dihub
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
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