Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor

Rawson, Frankie J.; Dodd, Nicholas; Abate, Wondwossen; Garrett, David J.; Yip, Nga-Chi; Fejer, Gyorgy; Downard, Alison J.; Baronian, Kim H.R.; Jackson, Simon K.; Mendes, Paula M.; Hicks, Jacqueline

Abstract

Herein, we report a highly sensitive electrocatalytic sensor-cell construct that can electrochemically communicate with the internal environment of immune cells (e.g., macrophages) via the selective monitoring of a particular reactive oxygen species (ROS), hydrogen peroxide. The sensor, which is based on vertically aligned single-walled carbon nanotubes functionalized with an osmium electrocatalyst, enabled the unprecedented detection of a local intracellular “pulse” of ROS on a short second time scale in response to bacterial endotoxin (lipopolysaccharide-LPS) stimulation. Our studies have shown that this initial pulse of ROS is dependent on NADPH oxidase (NOX) and toll like receptor 4 (TLR4). The results suggest that bacteria can induce a rapid intracellular pulse of ROS in macrophages that initiates the classical innate immune response of these cells to infection.

Citation

Rawson, F. J., Dodd, N., Abate, W., Garrett, D. J., Yip, N.-C., Fejer, G., Downard, A. J., Baronian, K. H., Jackson, S. K., Mendes, P. M., & Hicks, J. (2015). Fast, ultrasensitive detection of reactive oxygen species using a carbon nanotube based-electrocatalytic intracellular sensor. ACS Applied Materials and Interfaces, 7(42), https://doi.org/10.1021/acsami.5b06493

• Journal Article Type: Article
• Publication Date: Oct 28, 2015
• Deposit Date: Nov 27, 2015
• Publicly Available Date: Nov 27, 2015
• Journal: ACS Applied Materials and Interfaces
• Print ISSN: 1944-8244
• Electronic ISSN: 1944-8252
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 7
• Issue: 42
• DOI: https://doi.org/10.1021/acsami.5b06493
• Keywords: Intracellular Sensor, Electrocatalytic Sensor, Carbon Nanotubes, Reactive Oxygen Species, Hydrogen Peroxide, Macrophage Cells
• Public URL: https://nottingham-repository.worktribe.com/output/763052
• Publisher URL: http://pubs.acs.org/doi/abs/10.1021/acsami.5b06493