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The MYB36 transcription factor orchestrates Casparian strip formation

Kamiya, Takehiro; Borghi, Monica; Wang, Peng; Danku, John; Kalmbach, Lothar; Hosmani, Prashant S.; Naseer, Sadaf; Fujiwara, Toru; Geldner, Niko; Salt, David E.

Authors

Takehiro Kamiya

Monica Borghi

Peng Wang

John Danku

Lothar Kalmbach

Prashant S. Hosmani

Sadaf Naseer

Toru Fujiwara

Niko Geldner

David E. Salt



Abstract

The endodermis in roots acts as a selectivity filter for nutrient and water transport essential for growth and development. This selectivity is enabled by the formation of lignin-based Casparian strips. Casparian strip formation is initiated by the localization of the Casparian strip domain proteins (CASPs) in the plasma membrane, at the site where the Casparian strip will form. Localized CASPs recruit Peroxidase 64 (PER64), a Respiratory Burst Oxidase Homolog F, and Enhanced Suberin 1 (ESB1), a dirigent-like protein, to assemble the lignin polymerization machinery. However, the factors that control both expression of the genes encoding this biosynthetic machinery and its localization to the Casparian strip formation site remain unknown. Here, we identify the transcription factor, MYB36, essential for Casparian strip formation. MYB36 directly and positively regulates the expression of the Casparian strip genes CASP1, PER64, and ESB1. Casparian strips are absent in plants lacking a functional MYB36 and are replaced by ectopic lignin-like material in the corners of endodermal cells. The barrier function of Casparian strips in these plants is also disrupted. Significantly, ectopic expression of MYB36 in the cortex is sufficient to reprogram these cells to start expressing CASP1–GFP, correctly localize the CASP1–GFP protein to form a Casparian strip domain, and deposit a Casparian strip-like structure in the cell wall at this location. These results demonstrate that MYB36 is controlling expression of the machinery required to locally polymerize lignin in a fine band in the cell wall for the formation of the Casparian strip.

Citation

Kamiya, T., Borghi, M., Wang, P., Danku, J., Kalmbach, L., Hosmani, P. S., …Salt, D. E. (2015). The MYB36 transcription factor orchestrates Casparian strip formation. Proceedings of the National Academy of Sciences, 112(33), https://doi.org/10.1073/pnas.1507691112

Journal Article Type Article
Acceptance Date Jun 5, 2015
Publication Date Aug 18, 2015
Deposit Date Jan 26, 2017
Publicly Available Date Jan 26, 2017
Journal Proceedings of the National Academy of Sciences
Print ISSN 0027-8424
Electronic ISSN 1091-6490
Publisher National Academy of Sciences
Peer Reviewed Peer Reviewed
Volume 112
Issue 33
DOI https://doi.org/10.1073/pnas.1507691112
Keywords Casparian strip, transcription factor, lignin, endodermis, cell wall
Public URL http://eprints.nottingham.ac.uk/id/eprint/40112
Publisher URL http://www.pnas.org/content/112/33/10533
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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