Bunty Sharma
Metallocatanionic vesicle-mediated enhanced singlet oxygen generation and photodynamic therapy of cancer cells
Sharma, Bunty; Jain, Akhil; Pérez-García, Lluïsa; Watts, Julie A.; Rawson, Frankie J.; Chaudhary, Ganga Ram; Kaur, Gurpreet
Authors
Akhil Jain
Lluïsa Pérez-García
Julie A. Watts
Dr Frankie Rawson Frankie.Rawson@nottingham.ac.uk
ASSOCIATE PROFESSOR
Ganga Ram Chaudhary
Gurpreet Kaur
Abstract
In clinics, photodynamic therapy (PDT) is established as a non-invasive therapeutic modality for certain types of cancers and skin disease. However, due to poor water solubility, photobleaching, and the dark toxicity of photosensitizers (PSs), further developments are required to improve the efficiency of PDT. Herein, we report the role of metallocatanionic vesicles (MCVs) in enhancing the phototoxicity of methylene blue (MB) against cancer cells. These MCVs were prepared via a facile and quick solution-solution mixing method using a cationic single-chain metallosurfactant (FeCPC I) in combination with anionic sodium oleate (Na Ol). For singlet oxygen (1O2) generation and PDT studies, two fractions of FeCPC I : Na Ol, i.e., 30 : 70 (V37) and 70 : 30 (V73), were chosen based on their long-term stability in aqueous media. A cationic PS MB was loaded into these vesicles. The MB-loaded MCV 30 : 70 and 70 : 30 fractions enhanced the 1O2 generation by 0.10- and 0.40-fold, respectively, compared with MB alone. Upon illumination using a 650 nm laser, these MB-loaded V73 and V37 MCVs significantly decreased the metabolic activity of MCF-7 cells by ≤50% at a concentration of 0.75 μM. Furthermore, the SOSG assay revealed that the synthesized MCVs enhanced the intracellular 1O2 compared with MB alone. The MB-loaded V73 MCVs showed the highest 1O2-mediated membrane damage and cell-killing effect, as confirmed using the differential nuclear staining assay (DNS), which is attributed to the cellular uptake profile of the different MCV fractions. Altogether, this work shows the advantage of using these biocompatible and dual-charge MCVs as promising delivery vehicles that can enhance the 1O2 generation from the PS. This work suggests the future application of these Fe-MCVs in magnetically guided PDT.
Citation
Sharma, B., Jain, A., Pérez-García, L., Watts, J. A., Rawson, F. J., Chaudhary, G. R., & Kaur, G. (2022). Metallocatanionic vesicle-mediated enhanced singlet oxygen generation and photodynamic therapy of cancer cells. Journal of Materials Chemistry B, 10(13), 2160-2170. https://doi.org/10.1039/d2tb00011c
Journal Article Type | Article |
---|---|
Acceptance Date | Feb 18, 2022 |
Online Publication Date | Feb 18, 2022 |
Publication Date | Apr 7, 2022 |
Deposit Date | Oct 4, 2022 |
Publicly Available Date | Feb 19, 2023 |
Journal | Journal of Materials Chemistry B |
Print ISSN | 2050-750X |
Electronic ISSN | 2050-7518 |
Publisher | Royal Society of Chemistry |
Peer Reviewed | Peer Reviewed |
Volume | 10 |
Issue | 13 |
Pages | 2160-2170 |
DOI | https://doi.org/10.1039/d2tb00011c |
Keywords | General Materials Science; Biomedical Engineering; General Chemistry; General Medicine |
Public URL | https://nottingham-repository.worktribe.com/output/7578084 |
Publisher URL | https://pubs.rsc.org/en/content/articlelanding/2022/TB/D2TB00011C |
Files
JMB Manuscript
(1.3 Mb)
PDF
You might also like
Wireless electrical–molecular quantum signalling for cancer cell apoptosis
(2023)
Journal Article
Electric field responsive nanotransducers for glioblastoma
(2022)
Journal Article
Downloadable Citations
About Repository@Nottingham
Administrator e-mail: discovery-access-systems@nottingham.ac.uk
This application uses the following open-source libraries:
SheetJS Community Edition
Apache License Version 2.0 (http://www.apache.org/licenses/)
PDF.js
Apache License Version 2.0 (http://www.apache.org/licenses/)
Font Awesome
SIL OFL 1.1 (http://scripts.sil.org/OFL)
MIT License (http://opensource.org/licenses/mit-license.html)
CC BY 3.0 ( http://creativecommons.org/licenses/by/3.0/)
Powered by Worktribe © 2025
Advanced Search