Leigh A. Stoddart
Application of BRET to monitor ligand binding to GPCRs
Stoddart, Leigh A.; Johnstone, Elizabeth K.M.; Wheal, Amanda J.; Goulding, Joelle; Robers, Matthew B.; Machleidt, Thomas; Wood, Keith V.; Hill, Stephen J.; Pfleger, Kevin D.G.
Authors
Elizabeth K.M. Johnstone
Amanda J. Wheal
JOELLE GOULDING JOELLE.GOULDING@NOTTINGHAM.AC.UK
Senior Research Fellow
Matthew B. Robers
Thomas Machleidt
Keith V. Wood
STEPHEN HILL STEVE.HILL@NOTTINGHAM.AC.UK
Professor of Molecular Pharmacology
Kevin D.G. Pfleger
Abstract
Bioluminescence resonance energy transfer (BRET) is a well-established method for investigating protein-protein interactions. Here we present a BRET approach to monitor ligand binding to G protein–coupled receptors (GPCRs) on the surface of living cells made possible by the use of fluorescent ligands in combination with a bioluminescent protein (NanoLuc) that can be readily expressed on the N terminus of GPCRs.
Citation
Stoddart, L. A., Johnstone, E. K., Wheal, A. J., Goulding, J., Robers, M. B., Machleidt, T., …Pfleger, K. D. (in press). Application of BRET to monitor ligand binding to GPCRs. Nature Methods, 12, https://doi.org/10.1038/nmeth.3398
Journal Article Type | Article |
---|---|
Acceptance Date | Apr 4, 2015 |
Online Publication Date | Jun 1, 2015 |
Deposit Date | Jul 19, 2016 |
Publicly Available Date | Jul 19, 2016 |
Journal | Nature Methods |
Print ISSN | 1548-7091 |
Electronic ISSN | 1548-7105 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 12 |
DOI | https://doi.org/10.1038/nmeth.3398 |
Keywords | cell signalling, drug development, optical spectroscopy |
Public URL | https://nottingham-repository.worktribe.com/output/750676 |
Publisher URL | http://www.nature.com/nmeth/journal/v12/n7/full/nmeth.3398.html |
Contract Date | Jul 19, 2016 |
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