Elvira Ruslanovna Akhmetzyanova
Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage
Akhmetzyanova, Elvira Ruslanovna; Timofeeva, Anna Viktorovna; Sabirov, Davran Khudaishukurovich; Kostennikov, Alexander Alexandrovich; Rogozhin, Alexander Alexandrovich; James, Victoria; Arkhipova, S. S.; Rizvanov, Albert Anatolevich; Mukhamedshina, Yana Olegovna
Authors
Anna Viktorovna Timofeeva
Davran Khudaishukurovich Sabirov
Alexander Alexandrovich Kostennikov
Alexander Alexandrovich Rogozhin
VICTORIA JAMES VICTORIA.JAMES@NOTTINGHAM.AC.UK
Associate Professor
S. S. Arkhipova
Albert Anatolevich Rizvanov
Yana Olegovna Mukhamedshina
Abstract
Determination of the quantitative composition of phenotypically and morphologically different populations of resident microglia and infiltrating macrophages in spinal cord injury (SCI) of various degrees of severity could lead to much needed novel therapeutic interventions in neurotrauma. In this regard, we investigated the CD40 and TGF-β expressing populations of microglia/macrophages and their morphological states in a rat model of SCI of varying severity. We are the first to describe the annular-shaped microglia/macrophages, the morphology of which was formed due to the spatial orientation of the processes that form round or oval micro-territories, which include disintegrating myelin fibers. This type of cell morphology was found only in the injured spinal cord and mainly in the white matter. At the same time, an assessment of the number of annular-shaped microglia/macrophages and the diameter of micro-territories formed by their processes showed an elevation in these indicators as the severity of SCI increased. While we did not find significant quantitative changes in the populations of Iba1+/CD40+ and Iba1+/TGF-β+ microglia/macrophages with increased severity of SCI in the chronic period (60 dpi), we did determine changes in the expression of cytokines and mRNAs of genes-encoding microglial marker proteins, finding the greatest changes on days 7 and 14 after SCI between experimental groups with varying severity.
Citation
Akhmetzyanova, E. R., Timofeeva, A. V., Sabirov, D. K., Kostennikov, A. A., Rogozhin, A. A., James, V., …Mukhamedshina, Y. O. (2022). Increasing Severity of Spinal Cord Injury Results in Microglia/Macrophages With Annular-Shaped Morphology and No Change in Expression of CD40 and Tumor Growth Factor-β During the Chronic Post-injury Stage. Frontiers in Molecular Neuroscience, 14, Article 802558. https://doi.org/10.3389/fnmol.2021.802558
Journal Article Type | Article |
---|---|
Acceptance Date | Dec 28, 2020 |
Online Publication Date | Feb 24, 2022 |
Publication Date | Feb 24, 2022 |
Deposit Date | Mar 18, 2022 |
Publicly Available Date | Mar 18, 2022 |
Journal | Frontiers in Molecular Neuroscience |
Publisher | Frontiers Media |
Peer Reviewed | Peer Reviewed |
Volume | 14 |
Article Number | 802558 |
DOI | https://doi.org/10.3389/fnmol.2021.802558 |
Keywords | Cellular and Molecular Neuroscience; Molecular Biology |
Public URL | https://nottingham-repository.worktribe.com/output/7506573 |
Publisher URL | https://www.frontiersin.org/articles/10.3389/fnmol.2021.802558/full |
Files
fnmol-14-802558
(10.7 Mb)
PDF
Publisher Licence URL
https://creativecommons.org/licenses/by/4.0/
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