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Raised tone reveals ATP as a sympathetic neurotransmitter in the porcine mesenteric arterial bed

Shatarat, Amjad; Dunn, William R.; Ralevic, Vera

Authors

Amjad Shatarat

William R. Dunn

VERA RALEVIC vera.ralevic@nottingham.ac.uk
Associate Professor & Reader in Cardiovascular Sciences



Abstract

The relative importance of ATP as a functional sympathetic neurotransmitter in blood vessels has been shown to be increased when the level of preexisting vascular tone or pressure is increased, in studies carried out in rat mesenteric arteries. The aim of the present study was to determine whether tone influences the involvement of ATP as a sympathetic cotransmitter with noradrenaline in another species. We used the porcine perfused mesenteric arterial bed and porcine mesenteric large, medium and small arteries mounted for isometric tension recording, because purinergic cotransmission can vary depending on the size of the blood vessel. In the perfused mesenteric bed at basal tone, sympathetic neurogenic vasocontractile responses were abolished by prazosin, an α1- adrenoceptor antagonist, but there was no significant effect of α,β-methylene ATP, a P2X receptor-desensitizing agent. Submaximal precontraction of the mesenteric arterial bed with U46619, a thromboxane A2 mimetic, augmented the sympathetic neurogenic vasocontractile responses; under these conditions, both α,β-methylene ATP and prazosin attenuated the neurogenic responses. In the mesenteric large, medium and small arteries, prazosin attenuated the sympathetic neurogenic contractile responses under conditions of both basal and U46619-raised tone. α,β-Methylene ATP was effective in all of these arteries only under conditions of U46619- induced tone, causing a similar inhibition in all arteries, but had no significant effect on sympathetic neurogenic contractions at basal tone. These data show thatATP is a cotransmitter with noradrenaline in porcine mesenteric arteries; the purinergic component was revealed under conditions of partial precontraction, which is more relevant to physiological conditions.

Journal Article Type Article
Journal Purinergic Signalling
Print ISSN 1573-9538
Electronic ISSN 1573-9546
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 10
Issue 4
APA6 Citation Shatarat, A., Dunn, W. R., & Ralevic, V. (in press). Raised tone reveals ATP as a sympathetic neurotransmitter in the porcine mesenteric arterial bed. Purinergic Signalling, 10(4), https://doi.org/10.1007/s11302-014-9426-3
DOI https://doi.org/10.1007/s11302-014-9426-3
Keywords ATP, Cotransmission, Noradrenaline, P2X Receptor, Porcine Mesenteric Arteries, Sympathetic Nerves
Publisher URL http://link.springer.com/article/10.1007%2Fs11302-014-9426-3
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information The final publication is available at Springer via http://dx.doi.org/10.1007/s11302-014-9426-3

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Amjad paperV4 revised (v2).pdf (425 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf


Amjad Edited graphs (2).pdf (676 Kb)
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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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