Orchestration of ethylene and gibberellin signals determines primary root elongation in rice

Qin, Hua; Pandey, Bipin K; Li, Yuxiang; Huang, Guoqiang; Wang, Juan; Quan, Ruidang; Zhou, Jiahao; Zhou, Yun; Miao, Yuchen; Zhang, Dabing; Bennett, Malcolm J; Huang, Rongfeng

Abstract

Primary root growth in cereal crops is fundamental for early establishment of the seedling and grain yield. In young rice (Oryza sativa) seedlings, the primary root grows rapidly for 7-10 days after germination and then stops; however, the underlying mechanism determining primary root growth is unclear. Here, we report that the interplay of ethylene and gibberellin (GA) controls the orchestrated development of the primary root in young rice seedlings. Our analyses advance the knowledge that primary root growth is maintained by higher ethylene production, which lowers bioactive GA contents. Further investigations unraveled that ethylene signaling transcription factor ETHYLENE INSENSITIVE3-LIKE 1 (OsEIL1) activates the expression of the GA metabolism genes GIBBERELLIN 2-OXIDASE 1 (OsGA2ox1), OsGA2ox2, OsGA2ox3, and OsGA2ox5, thereby deactivating GA activity, inhibiting cell proliferation in the root meristem, and ultimately gradually inhibiting primary root growth. Mutation in OsGA2ox3 weakened ethylene-induced GA inactivation and reduced the ethylene sensitivity of the root. Genetic analysis revealed that OsGA2ox3 functions downstream of OsEIL1. Taken together, we identify a molecular pathway impacted by ethylene during primary root elongation in rice and provide insight into the coordination of ethylene and GA signals during root development and seedling establishment.

Citation

Qin, H., Pandey, B. K., Li, Y., Huang, G., Wang, J., Quan, R., Zhou, J., Zhou, Y., Miao, Y., Zhang, D., Bennett, M. J., & Huang, R. (2022). Orchestration of ethylene and gibberellin signals determines primary root elongation in rice. Plant Cell, 34(4), 1273-1288. https://doi.org/10.1093/plcell/koac008

• Journal Article Type: Article
• Acceptance Date: Jan 6, 2022
• Online Publication Date: Jan 11, 2022
• Publication Date: 2022-04
• Deposit Date: Feb 28, 2022
• Publicly Available Date: Jan 12, 2023
• Journal: Plant Cell
• Print ISSN: 1040-4651
• Electronic ISSN: 1532-298X
• Publisher: American Society of Plant Biologists
• Peer Reviewed: Peer Reviewed
• Volume: 34
• Issue: 4
• Pages: 1273-1288
• DOI: https://doi.org/10.1093/plcell/koac008
• Keywords: Cell Biology; Plant Science
• Public URL: https://nottingham-repository.worktribe.com/output/7349354
• Publisher URL: https://academic.oup.com/plcell/advance-article-abstract/doi/10.1093/plcell/koac008/6503509?redirectedFrom=fulltext