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Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs

Graham, Neil S.; Hammond, John P.; Lysenko, Artem; Mayes, Sean; Ó Lochlainn, Seosamh; Blasco, Bego; Bowen, Helen C.; Rawlings, Chris J.; Rios, Juan J.; Welham, Susan; Carion, Pierre W.C.; Dupuy, Lionel X.; King, Graham J.; White, Philip J.; Broadley, Martin R.

Authors

NEIL GRAHAM neil.graham@nottingham.ac.uk
Senior Research Fellow

John P. Hammond

Artem Lysenko

SEAN MAYES SEAN.MAYES@NOTTINGHAM.AC.UK
Associate Professor

Seosamh Ó Lochlainn

Bego Blasco

Helen C. Bowen

Chris J. Rawlings

Juan J. Rios

Susan Welham

Pierre W.C. Carion

Lionel X. Dupuy

Graham J. King

Philip J. White



Abstract

Although Ca transport in plants is highly complex, the overexpression of vacuolar Ca2+ transporters in crops is a promising new technology to improve dietary Ca supplies through biofortification. Here, we sought to identify novel targets for increasing plant Ca accumulation using genetical and comparative genomics. Expression quantitative trait locus (eQTL) mapping to 1895 cis- and 8015 trans-loci were identified in shoots of an inbred mapping population of Brassica rapa (IMB211 x R500); 23 cis- and 948 trans-eQTLs responded specifically to altered Ca supply. eQTLs were screened for functional significance using a large database of shoot Ca concentration phenotypes of Arabidopsis thaliana. From 31 Arabidopsis gene identifiers tagged to robust shoot Ca concentration phenotypes, 21 mapped to 27 B. rapa eQTLs, including orthologs of the Ca2+ transporters At-CAX1 and At-ACA8. Two of three independent missense mutants of BraA.cax1a, isolated previously by targeting induced local lesions in genomes, have allele-specific shoot Ca concentration phenotypes compared with their segregating wild types. BraA.CAX1a is a promising target for altering the Ca composition of Brassica, consistent with prior knowledge from Arabidopsis. We conclude that multiple-environment eQTL analysis of complex crop genomes combined with comparative genomics is a powerful technique for novel gene identification/prioritization.

Journal Article Type Article
Journal Plant Cell
Print ISSN 1040-4651
Electronic ISSN 1532-298X
Publisher American Society of Plant Biologists
Peer Reviewed Peer Reviewed
Volume 26
Issue 7
APA6 Citation Graham, N. S., Hammond, J. P., Lysenko, A., Mayes, S., Ó Lochlainn, S., Blasco, B., …Broadley, M. R. (in press). Genetical and comparative genomics of Brassica under altered Ca supply identifies Arabidopsis Ca-transporter orthologs. Plant Cell, 26(7),
Publisher URL http://www.plantcell.org/content/26/7/2818
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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