Imaging early endothelial inflammation following stroke by core shell silica superparamagnetic glyconanoparticles that target selectin

Farr, Tracy D.; Lai, Chian-Hui; Gr�nstein, Dan; Orts-Gil, Guillermo; Wang, Cheng-Chung; Boehm-Sturm, Philipp; Seeberger, Peter H.; Harms, Christoph

Abstract

Activation of the endothelium is a pivotal first step for leukocyte migration into the diseased brain. Consequently, imaging this activation process is highly desirable. We synthesized carbohydrate-functionalized magnetic nanoparticles that bind specifically to the endothelial transmembrane inflammatory proteins E and P selectin. Magnetic resonance imaging revealed that the targeted nanoparticles accumulated in the brain vasculature following acute administration into a clinically relevant animal model of stroke, though increases in selectin expression were observed in both brain hemispheres. Nonfunctionalized naked particles also appear to be a plausible agent to target the ischemic vasculature. The importance of these findings is discussed regarding the potential for translation into the clinic.

• Journal Article Type: Article
• Publication Date: Feb 25, 2014
• Deposit Date: Apr 15, 2016
• Publicly Available Date: Apr 15, 2016
• Journal: Nano Letters
• Print ISSN: 1530-6984
• Electronic ISSN: 1530-6992
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 4
• DOI: https://doi.org/10.1021/nl500388h
• Keywords: Glyco-magnetic nanoparticle; MRI; Stroke; Selection; Nano-medicine
• Public URL: https://nottingham-repository.worktribe.com/output/722837
• Publisher URL: http://dx.doi.org/10.1021/nl500388h
• Additional Information: This document is the unedited Author’s version of a Submitted Work that was subsequently accepted for publication in Nano Letters, copyright © American Chemical Society after peer review. To access the final edited and published work see http://dx.doi.org/10.1021/nl500388h