Disordered Protein Stabilization by Co-Assembly of Short Peptides Enables Formation of Robust Membranes

Ghosh, Moumita; Majkowska, Anna; Mirsa, Rajkumar; Bera, Santu; Rodríguez-Cabello, José Carlos; Mata, Alvaro; Adler-Abramovich, Lihi

Abstract

Molecular self-assembly is a spontaneous natural process resulting in highly ordered nano to microarchitectures. We report temperature-independent formation of robust stable membranes obtained by the spontaneous interaction of intrinsically disordered elastin-like polypeptides (ELPs) with short aromatic peptides at temperatures both below and above the conformational transition temperature of the ELPs. The membranes are stable over time and display durability over a wide range of parameters including temperature, pH, and ultrasound energy. The morphology and composition of the membranes were analyzed using microscopy. These robust structures support preosteoblast cell adhesion and proliferation as well as pH-dependent cargo release. Simple noncovalent interactions with short aromatic peptides can overcome conformational restrictions due to the phase transition to facilitate the formation of complex bioactive scaffolds that are stable over a wide range of environmental parameters. This approach offers novel possibilities for controlling the conformational restriction of intrinsically disordered proteins and using them in the design of new materials.

Citation

Ghosh, M., Majkowska, A., Mirsa, R., Bera, S., Rodríguez-Cabello, J. C., Mata, A., & Adler-Abramovich, L. (2022). Disordered Protein Stabilization by Co-Assembly of Short Peptides Enables Formation of Robust Membranes. ACS Applied Materials and Interfaces, 14(1), 464-473. https://doi.org/10.1021/acsami.1c22136

• Journal Article Type: Article
• Acceptance Date: Nov 29, 2021
• Online Publication Date: Dec 23, 2021
• Publication Date: Jan 12, 2022
• Deposit Date: Jan 13, 2022
• Publicly Available Date: Dec 24, 2022
• Journal: ACS Applied Materials and Interfaces
• Print ISSN: 1944-8244
• Electronic ISSN: 1944-8252
• Publisher: American Chemical Society
• Peer Reviewed: Peer Reviewed
• Volume: 14
• Issue: 1
• Pages: 464-473
• DOI: https://doi.org/10.1021/acsami.1c22136
• Keywords: General Materials Science
• Public URL: https://nottingham-repository.worktribe.com/output/7225456
• Publisher URL: https://pubs.acs.org/doi/10.1021/acsami.1c22136