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Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis

Chan, Xin Yue; Chang, Chien Yi; Hong, Kar Wai; Tee, Kok Keng; Yin, Wai Fong; Chan, Kok Gan

Authors

Xin Yue Chan

Chien Yi Chang

Kar Wai Hong

Kok Keng Tee

Wai Fong Yin

Kok Gan Chan

Abstract

Background
Serratia marcescens is an opportunistic bacterial pathogen with broad range of host ranging from vertebrates, invertebrates and plants. S. marcescens strain W2.3 was isolated from a diseased tilapia fish and it was suspected to be the causal agent for the fish disease as virulence genes were found within its genome. In this study, for the first time, the genome sequences of S. marcescens strain W2.3 were sequenced using the Illumina MiSeq platform.

Result
Several virulent factors of S. marcescens such as serrawettin, a biosurfactant, has been reported to be regulated by N-acyl homoserine lactone (AHL)-based quorum sensing (QS). In our previous studies, an unusual AHL with long acyl side chain was detected from this isolate suggesting the possibility of novel virulence factors regulation. This evokes our interest in the genome of this bacterial strain and hereby we present the draft genome of S. marcescens W2.3, which carries the serrawettin production gene, swrA and the AHL-based QS transcriptional regulator gene, luxR which is an orphan luxR.

Conclusion
With the availability of the whole genome sequences of S. marcescens W2.3, this will pave the way for the study of the QS-mediated genes expression in this bacterium.

Journal Article Type Article
Publication Date Oct 22, 2013
Journal Gut Pathogens
Electronic ISSN 1757-4749
Publisher Humana Press
Peer Reviewed Peer Reviewed
Volume 5
Issue Octobe
Article Number 5
Institution Citation Chan, X. Y., Chang, C. Y., Hong, K. W., Tee, K. K., Yin, W. F., & Chan, K. G. (2013). Insights of biosurfactant producing Serratia marcescens strain W2.3 isolated from diseased tilapia fish: a draft genome analysis. Gut Pathogens, 5(Octobe), doi:10.1186/1757-4749-5-29
DOI https://doi.org/10.1186/1757-4749-5-29
Keywords Serratia marcescens, Biosurfactant, Serrawettin, Quorum sensing, N-acyl homoserine lactone, swrA, Next
generation sequencing technology
Publisher URL http://www.gutpathogens.com/content/5/1/29
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0




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