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Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI

Lilburn, David M.L.; Hughes-Riley, Theodore; Six, Joseph S.; Stupic, Karl F.; Shaw, Dominick E.; Pavlovskaya, Galina E.; Meersmann, Thomas

Authors

David M.L. Lilburn

Theodore Hughes-Riley

Joseph S. Six

Karl F. Stupic

Dominick E. Shaw

Galina E. Pavlovskaya

Thomas Meersmann



Abstract

Ex vivo rodent lung models are explored for physiological measurements of respiratory function with hyperpolarized (hp) 129Xe MRI. It is shown that excised lung models allow for simplification of the technical challenges involved and provide valuable physiological insights that are not feasible using in vivo MRI protocols. A custom designed breathing apparatus enables MR images of gas distribution on increasing ventilation volumes of actively inhaled hp 129Xe. Straightforward hp 129Xe MRI protocols provide residual lung volume (RV) data and permit for spatially resolved tracking of small hp 129Xe probe volumes during the inhalation cycle. Hp 129Xe MRI of lung function in the excised organ demonstrates the persistence of post mortem airway responsiveness to intravenous methacholine challenges. The presented methodology enables physiology of lung function in health and disease without additional regulatory approval requirements and reduces the technical and logistical challenges with hp gas MRI experiments. The post mortem lung functional data can augment histological measurements and should be of interest for drug development studies.

Journal Article Type Article
Publication Date Aug 30, 2013
Journal PLoS ONE
Electronic ISSN 1932-6203
Publisher Public Library of Science
Peer Reviewed Peer Reviewed
Volume 8
Issue 8
Article Number e73468
APA6 Citation Lilburn, D. M., Hughes-Riley, T., Six, J. S., Stupic, K. F., Shaw, D. E., Pavlovskaya, G. E., & Meersmann, T. (2013). Validating excised rodent lungs for functional hyperpolarized xenon-129 MRI. PLoS ONE, 8(8), doi:10.1371/journal.pone.0073468
DOI https://doi.org/10.1371/journal.pone.0073468
Publisher URL http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0073468
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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