Peptic digestion of beef myofibrils is modified by prior marination

Patel, Nash; Welham, Simon J.M.

Abstract

Background: Preparatory steps such as seasoning, marination, and cooking may induce changes in meat
which affects the ability of the stomach to adequately digest it. This may result in peptide chains reaching the
colon intact where resident bacteria ferment them resulting in the formation of putative carcinogenic phenolic
by-products.
Objective: In this study, we set out to determine whether peptic digestion of beef myofibrils was influenced by
prior marination.
Design: Cubes of sirloin stewing steak were marinated in balsamic vinegar or left untreated at 48C overnight.
Samples were oven cooked and myofibrils were extracted. Myofibrils were subject to proteolytic digestion
with pepsin and digestion products analysed spectrophotometrically and with gel electrophoresis.
Results: Both marination in balsamic vinegar and cooking significantly reduced the yield of myofibrils from
shop-purchased beef (PB0.05). Digestion progressed in all samples as a function of time (PB0.01), varying
depending on prior treatment. Marination induced resistance to the digestive effect of pepsin during the early
to mid-phase of digestion, and we identified a protein band of 150 kDa which was protected from peptic
digestion in samples which had been marinated and cooked, but not in any other groups.
Conclusions: Pre-treatment of meat prior to cooking may influence specific peptides such that they become
more resistant to the digestive actions of pepsin.
Keywords: marination; digestibility; acetic acid; colon cancer; myofibril; cooking; steak

Citation

Patel, N., & Welham, S. J. (2013). Peptic digestion of beef myofibrils is modified by prior marination. Food and Nutrition Research, 57(20294), https://doi.org/10.3402/fnr.v57i0.20294

• Journal Article Type: Article
• Publication Date: May 23, 2013
• Deposit Date: Apr 15, 2014
• Publicly Available Date: Apr 15, 2014
• Journal: Food and Nutrition Research
• Print ISSN: 1654-6628
• Electronic ISSN: 1654-661X
• Publisher: Taylor & Francis Open Access
• Peer Reviewed: Peer Reviewed
• Volume: 57
• Issue: 20294
• DOI: https://doi.org/10.3402/fnr.v57i0.20294
• Public URL: https://nottingham-repository.worktribe.com/output/715017
• Publisher URL: http://www.foodandnutritionresearch.net/index.php/fnr/article/view/20294