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A comparative study of adhesion of melanoma and breast cancer cells to blood and lymphatic endothelium

Safuan, Sabreena; Storr, Sarah J.; Patel, Poulam M.; Martin, Stewart G.

Authors

Sabreena Safuan

Sarah J. Storr

Poulam M. Patel

Stewart G. Martin



Abstract

Background: Lymphovascular invasion (LVI) is an important step in the metastatic cascade; tumor cell migration
and adhesion to blood and lymphatic vessels is followed by invasion through the vessel wall and subsequent
systemic spread. Although primary breast cancers and melanomas have rich blood vascular networks, LVI is
predominately lymphatic in nature. Whilst the adhesion of tumor cells to blood endothelium has been extensively
investigated, there is a paucity of information on tumor cell adhesion to lymphatic endothelium.
Methods and Results: Breast cancer (MDA-MB-231 and MCF7) and melanoma (MeWo and SKMEL-30) cell
adhesion to lymphatic (hTERT-LEC and HMVEC dLy Neo) and blood (HUVEC and hMEC-1) endothelial cells
were assessed using static adhesion assays. The effect of inflammatory conditions, tumor necrosis factor-a
(TNF-a) stimulation of endothelial and tumor cells, on the adhesive process was also examined. In addition,
the effects of TNF-a stimulation on tumor cell migration was investigated using haplotaxis (scratch wound)
assays. Breast cancer and melanoma cells exhibited higher levels of adhesion to blood compared to lymphatic
endothelial cells ( p < 0.001). TNF-a stimulation of endothelial cells, or of tumor cells alone, did not significantly
alter tumor–endothelial cell adhesion or patterns.When both tumor and endothelial cells were stimulated with
TNF-a, a significant increase in adhesion was observed ( p < 0.01), which was notably higher in the lymphatic
cell models ( p < 0.001). TNF-a-stimulation of all tumor cell lines significantly increased their migration rate
( p < 0.01).
Conclusions: Results suggest that metastasis resultant from lymphatic vessel-tumor cell adhesion may be
modulated by cytokine stimulation, which could represent an important therapeutic target in breast cancer and
melanoma.

Journal Article Type Article
Publication Date Dec 16, 2012
Journal Lymphatic Research and Biology
Print ISSN 1539-6851
Electronic ISSN 1539-6851
Publisher Mary Ann Liebert
Peer Reviewed Peer Reviewed
Volume 10
Issue 4
Institution Citation Safuan, S., Storr, S. J., Patel, P. M., & Martin, S. G. (2012). A comparative study of adhesion of melanoma and breast cancer cells to blood and lymphatic endothelium. Lymphatic Research and Biology, 10(4), doi:10.1089/lrb.2012.0007
DOI https://doi.org/10.1089/lrb.2012.0007
Publisher URL http://online.liebertpub.com/doi/abs/10.1089/lrb.2012.0007
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





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