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Calpain system protein expression in carcinomas of the pancreas, bile duct and ampulla

Storr, Sarah J.; Zaitoun, Abed M.; Arora, Arvind; Durrant, Lindy G.; Lobo, Dileep N.; Madhusudan, Srinivasan; Martin, Stewart G.

Authors

Sarah J. Storr

Abed M. Zaitoun

Arvind Arora

Lindy G. Durrant

Dileep N. Lobo

Srinivasan Madhusudan

Stewart G. Martin



Abstract

Background: Pancreatic cancer, including cancer of the ampulla of Vater and bile duct, is very aggressive and has a
poor five year survival rate; improved methods of patient stratification are required.
Methods: We assessed the expression of calpain-1, calpain-2 and calpastatin in two patient cohorts using
immunohistochemistry on tissue microarrays. The first cohort was composed of 68 pancreatic adenocarcinomas
and the second cohort was composed of 120 cancers of the bile duct and ampulla.
Results: In bile duct and ampullary carcinomas an association was observed between cytoplasmic calpastatin
expression and patient age (P = 0.036), and between nuclear calpastatin expression and increased tumour stage
(P = 0.026) and the presence of vascular invasion (P = 0.043). In pancreatic cancer, high calpain-2 expression was
significantly associated with improved overall survival (P = 0.036), which remained significant in multivariate
Cox-regression analysis (hazard ratio = 0.342; 95% confidence interva l = 0.157-0.741; P = 0.007). In cancers of the
bile duct and ampulla, low cytoplasmic expression of calpastatin was significantly associated with poor overall
survival (P = 0.012), which remained significant in multivariate Cox-regression analysis (hazard ratio = 0.595; 95%
confidence interval = 0.365-0.968; P = 0.037).
Conclusion: The results suggest that calpain-2 and calpastatin expression is important in pancreatic cancers,
influencing disease progression. The findings of this study warrant a larger follow-up study.
Keywords: Calpain, Calpastatin, Pancreas, Ampulla, Bile duct, Cancer

Journal Article Type Article
Publication Date Nov 9, 2012
Journal BMC Cancer
Electronic ISSN 1471-2407
Publisher Springer Verlag
Peer Reviewed Peer Reviewed
Volume 12
Issue 511
Institution Citation Storr, S. J., Zaitoun, A. M., Arora, A., Durrant, L. G., Lobo, D. N., Madhusudan, S., & Martin, S. G. (2012). Calpain system protein expression in carcinomas of the pancreas, bile duct and ampulla. BMC Cancer, 12(511), doi:10.1186/1471-2407-12-511
DOI https://doi.org/10.1186/1471-2407-12-511
Publisher URL http://www.biomedcentral.com/1471-2407/12/511/
Copyright Statement Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0
Additional Information Copy of Creative Commons Attribution License must accompany any deposit.

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Copyright Statement
Copyright information regarding this work can be found at the following address: http://creativecommons.org/licenses/by/4.0





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