Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy

Fareed, Khaleel R.; Soomro, Irshad N.; Hameed, Khalid; Arora, Arvind; Lobo, Dileep N.; Parsons, Simon L.; Madhusudan, Srinivasan

Abstract

AIM: To examine cytokeratin-18 (CK-18) and caspase-cleaved CK-18 expression in tumours and correlate with clinicopathological outcomes including tumour regression grade (TRG) response.

METHODS: Formalin-fixed human gastro-oesophageal cancers were constructed into tissue microarrays. The first set consisted of 122 gastric/gastro-oesophageal cancer cases not exposed to neoadjuvant chemotherapy and the second set consisted of 97 gastric/gastro-oesophageal cancer cases exposed to pre-operative platinum-based chemotherapy. Expression of CK-18 and caspase-cleaved CK-18 was investigated using immunohistochemistry.

RESULTS: CK18 was commonly expressed in gastro-oesophageal tumours (92.6%). Fifty-six point seven percent of tumours previously exposed to neoadjuvant chemotherapy were positive for caspase-cleaved CK-18 expression compared to only 24.6% of tumours not previously exposed to neoadjuvant chemotherapy (P = 0.009). In patients who received neoadjuvant chemotherapy, caspase-cleaved cytokeratin-18 expression correlated with favourable TRG response (TRG 1, 2 or 3, P = 0.043).

CONCLUSION: This is the largest study to date of CK-18 and caspase-cleaved CK-18 expression in gastro-oesophageal tumours. We provide the first evidence that caspase-cleaved CK-18 predicts tumour regression with neoadjuvant chemotherapy.

Citation

Fareed, K. R., Soomro, I. N., Hameed, K., Arora, A., Lobo, D. N., Parsons, S. L., & Madhusudan, S. (2012). Caspase-cleaved cytokeratin-18 and tumour regression in gastro-oesophageal adenocarcinomas treated with neoadjuvant chemotherapy. World Journal of Gastroenterology, 18(16), https://doi.org/10.3748/wjg.v18.i16.1915

• Journal Article Type: Article
• Publication Date: Apr 28, 2012
• Deposit Date: Apr 1, 2014
• Publicly Available Date: Apr 1, 2014
• Journal: World Journal of Gastroenterology
• Print ISSN: 1007-9327
• Electronic ISSN: 2219-2840
• Publisher: Baishideng Publishing Group
• Peer Reviewed: Peer Reviewed
• Volume: 18
• Issue: 16
• DOI: https://doi.org/10.3748/wjg.v18.i16.1915
• Public URL: https://nottingham-repository.worktribe.com/output/709857
• Publisher URL: http://www.wjgnet.com/1007-9327/abstract/v18/i16/1915.htm