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Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin

Rashidi, Hassan; Strohbuecker, Stephanie; Jackson, Leigh; Kalra, Spandan; Blake, Alexander J.; France, Louise; Tufarelli, Cristina; Sottile, Virginie

Authors

Hassan Rashidi

Stephanie Strohbuecker

Leigh Jackson

Spandan Kalra

Alexander J. Blake

Louise France

Cristina Tufarelli

Virginie Sottile

Abstract

Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling.

Journal Article Type Article
Publication Date Nov 23, 2011
Journal Cells Tissues Organs
Print ISSN 1422-6405
Electronic ISSN 1422-6421
Publisher Karger Publishers
Peer Reviewed Peer Reviewed
Volume 195
Issue 6
Institution Citation Rashidi, H., Strohbuecker, S., Jackson, L., Kalra, S., Blake, A. J., France, L., …Sottile, V. (2011). Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin. Cells Tissues Organs, 195(6), doi:10.1159/000329861
DOI https://doi.org/10.1159/000329861
Publisher URL https://www.karger.com/Article/Abstract/329861
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf

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