Hassan Rashidi
Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin
Rashidi, Hassan; Strohbuecker, Stephanie; Jackson, Leigh; Kalra, Spandan; Blake, Alexander J.; France, Louise; Tufarelli, Cristina; Sottile, Virginie
Authors
Stephanie Strohbuecker
Leigh Jackson
Spandan Kalra
Alexander J. Blake
Louise France
Cristina Tufarelli
Virginie Sottile
Abstract
Bone marrow-derived mesenchymal stem cells (MSCs) are widely used as a cellular model of bone formation, and can mineralize in vitro in response to osteogenic medium (OM). It is unclear, however, whether this property is specific to cells of mesenchymal origin. We analysed the OM response in 3 non-osteogenic lines, HEK293, HeLa and NTera, compared to MSCs. Whereas HEK293 cells failed to respond to OM conditions, the 2 carcinoma-derived lines NTera and HeLa deposited a calcium phosphate mineral comparable to that present in MSC cultures. However, unlike MSCs, HeLa and NTera cultures did so in the absence of dexamethasone. This discrepancy was confirmed, as bone morphogenetic protein inhibition obliterated the OM response in MSCs but not in HeLa or NTera, indicating that these 2 models can deposit mineral through a mechanism independent of established dexamethasone or bone morphogenetic protein signalling.
Citation
Rashidi, H., Strohbuecker, S., Jackson, L., Kalra, S., Blake, A. J., France, L., …Sottile, V. (2011). Differences in the pattern and regulation of mineral deposition in human cell lines of osteogenic and non-osteogenic origin. Cells Tissues Organs, 195(6), https://doi.org/10.1159/000329861
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 7, 2011 |
Publication Date | Nov 23, 2011 |
Deposit Date | Feb 22, 2018 |
Publicly Available Date | Mar 29, 2024 |
Journal | Cells Tissues Organs |
Print ISSN | 1422-6405 |
Electronic ISSN | 1422-6421 |
Publisher | Karger Publishers |
Peer Reviewed | Peer Reviewed |
Volume | 195 |
Issue | 6 |
DOI | https://doi.org/10.1159/000329861 |
Public URL | https://nottingham-repository.worktribe.com/output/708652 |
Publisher URL | https://www.karger.com/Article/Abstract/329861 |
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