Skip to main content

Research Repository

See what's under the surface

Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif

Egan, Sharon A.; Kurian, Dominic; Ward, Philip N.; Hunt, Lawrence; Leigh, James A.

Authors

Sharon A. Egan

Dominic Kurian

Philip N. Ward

Lawrence Hunt

James A. Leigh



Abstract

Sortase (a transamidase) has been shown to be responsible for the covalent attachment of proteins to the bacterial cell wall. Anchoring is effected on secreted proteins containing a specific cell wall motif toward their C-terminus; that for sortase A (SrtA) in Gram-positive bacteria often incorporates the sequence LPXTG. Such surface proteins are often characterized as virulence determinants and play important roles during the establishment and persistence of infection. Intramammary infection with Streptococcus uberis is a common cause of bovine mastitis, which impacts on animal health and welfare and the economics of milk production. Comparison of stringently produced cell wall fractions from S. uberis and an isogenic mutant strain lacking SrtA permitted identification of 9 proteins likely to be covalently anchored at the cell surface. Analysis of these sequences implied the presence of two anchoring motifs for S. uberis, the classical LPXTG motif and an additional LPXXXD motif.

Journal Article Type Article
Publication Date Dec 29, 2009
Journal Journal of Proteome Research
Print ISSN 1535-3893
Electronic ISSN 1535-3907
Publisher American Chemical Society
Peer Reviewed Peer Reviewed
Volume 9
Issue 2
Institution Citation Egan, S. A., Kurian, D., Ward, P. N., Hunt, L., & Leigh, J. A. (2009). Identification of sortase A (SrtA) substrates in Streptococcus uberis: evidence for an additional hexapeptide (LPXXXD) sorting motif. Journal of Proteome Research, 9(2), doi:10.1021/pr901025w
DOI https://doi.org/10.1021/pr901025w
Keywords Sortase A, LPXTG, Cell wall proteins, Mastitis, Streptococcus uberis
Publisher URL http://pubs.acs.org/doi/abs/10.1021/pr901025w
Copyright Statement Copyright information regarding this work can be found at the following address: http://eprints.nottingh.../end_user_agreement.pdf
Additional Information This document is the unedited author's version of a submitted work that was subsequently accepted for publication in the Journal of Proteome Research, copyright American Chemical Society. To access the final edited and published work see http://pubs.acs.org/doi/abs/10.1021/pr901025w

Files

Sortase%20Cell%20wall%20manuscript[1].pdf (421 Kb)
PDF

Copyright Statement
Copyright information regarding this work can be found at the following address: http://eprints.nottingham.ac.uk/end_user_agreement.pdf





Downloadable Citations