The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16

Ehsaan, Muhammad; Baker, Jonathan; Kovács, Katalin; Malys, Naglis; Minton, Nigel P.

Abstract

Cupriavidus necator H16 can convert CO2 into industrial chemicals and fuels. To facilitate its engineering, we designed, built and tested the pMTL70000 modular plasmids comprising standardised Cupriavidus and E. coli replicons, selectable markers and application specific modules. Plasmids were characterised in terms of transmissibility, stability, copy number and compatibility.

Citation

Ehsaan, M., Baker, J., Kovács, K., Malys, N., & Minton, N. P. (2021). The pMTL70000 modular, plasmid vector series for strain engineering in Cupriavidus necator H16. Journal of Microbiological Methods, 189, Article 106323. https://doi.org/10.1016/j.mimet.2021.106323

• Journal Article Type: Article
• Acceptance Date: Sep 6, 2021
• Online Publication Date: Sep 8, 2021
• Publication Date: Oct 1, 2021
• Deposit Date: Sep 14, 2021
• Publicly Available Date: Sep 14, 2021
• Journal: Journal of Microbiological Methods
• Print ISSN: 0167-7012
• Electronic ISSN: 1872-8359
• Publisher: Elsevier BV
• Peer Reviewed: Peer Reviewed
• Volume: 189
• Article Number: 106323
• DOI: https://doi.org/10.1016/j.mimet.2021.106323
• Public URL: https://nottingham-repository.worktribe.com/output/6238666
• Publisher URL: https://www.sciencedirect.com/science/article/pii/S0167701221001913