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In vitro Effects of Biologically Active Vitamin D on Myogenesis: A Systematic Review

Alliband, Kathryn H.; Kozhevnikova, Sofia V.; Parr, Tim; Jethwa, Preeti H.; Brameld, John M.

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Kathryn H. Alliband

Sofia V. Kozhevnikova

Professor of Nutritional Biochemistry

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Professor of Nutritional Biochemistry


Vitamin D (VD) deficiency is associated with muscle weakness. A reduction in the incidence of falls in the elderly following VD supplementation and identification of the VD receptor within muscle cells suggests a direct effect of VD on muscle, but little is known about the underlying mechanisms. Here we systematically searched the literature to identify effects of active VD [1,25(OH)2D3] on skeletal muscle myogenesis in vitro, with no restriction on year of publication. Eligibility was assessed by strict inclusion/exclusion criteria and agreed by two independent investigators. Twelve relevant pa-pers were identified using four different cell types (C2C12, primary mouse satellite cells, primary chick myoblasts, and primary human myoblasts) and a range of myogenic markers (myoD, myogenin, creatine kinase, myosin heavy chain, and myotube size). A clear inhibitory effect of 1,25(OH)2D3 on proliferation was reported, while the effects on the different stages of differentiation were less consistent probably due to variation in cell type, time points and doses of 1,25(OH)2D3 used. However, myotube size was consistently increased by 1,25(OH)2D3. Overall, the evidence suggests that 1,25(OH)2D3 inhibits proliferation and promotes differentiation of myoblasts, but future studies should use time courses to gain a clearer understanding.

Journal Article Type Review
Acceptance Date Aug 6, 2021
Online Publication Date Sep 9, 2021
Publication Date Sep 9, 2021
Deposit Date Aug 9, 2021
Publicly Available Date Aug 11, 2021
Journal Frontiers in Physiology
Electronic ISSN 1664-042X
Peer Reviewed Peer Reviewed
Volume 12
Article Number 736708
Public URL
Publisher URL


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