A pipeline for making 31P NMR accessible for small- and large-scale lipidomics studies

Furse, Samuel; Williams, Huw E. L.; Watkins, Adam J.; Virtue, Samuel; Vidal-Puig, Antonio; Amarsi, Risha; Charalambous, Marika; Koulman, Albert

Abstract

Detailed molecular analysis is of increasing importance in research into the regulation of biochemical pathways, organismal growth and disease. Lipidomics in particular is increasingly sought after as it provides insight into molecular species involved in energy storage, signalling and fundamental cellular structures. This has led to the use of a range of tools and techniques to acquire lipidomics data. 31P NMR for lipidomics offers well-resolved head group/lipid class analysis, structural data that can be used to inform and strengthen interpretation of mass spectrometry data and part of a priori structural determination. In the present study, we codify the use of 31P NMR for lipidomics studies to make the technique more accessible to new users and more useful for a wider range of questions. The technique can be used in isolation (phospholipidomics) or as a part of determining lipid composition (lipidomics). We describe the process from sample extraction to data processing and analysis. This pipeline is important because it allows greater thoroughness in lipidomics studies and increases scope for answering scientific questions about lipid-containing systems.

Citation

Furse, S., Williams, H. E. L., Watkins, A. J., Virtue, S., Vidal-Puig, A., Amarsi, R., …Koulman, A. (2021). A pipeline for making 31P NMR accessible for small- and large-scale lipidomics studies. Analytical and Bioanalytical Chemistry, 413(19), 4763-4773. https://doi.org/10.1007/s00216-021-03430-4

• Journal Article Type: Article
• Acceptance Date: May 22, 2021
• Online Publication Date: Jul 12, 2021
• Publication Date: 2021-08
• Deposit Date: Jul 22, 2021
• Publicly Available Date: Jul 27, 2021
• Journal: Analytical and Bioanalytical Chemistry
• Print ISSN: 1618-2642
• Electronic ISSN: 1618-2650
• Publisher: Springer Science and Business Media LLC
• Peer Reviewed: Peer Reviewed
• Volume: 413
• Issue: 19
• Pages: 4763-4773
• DOI: https://doi.org/10.1007/s00216-021-03430-4
• Keywords: Biochemistry; Analytical Chemistry
• Public URL: https://nottingham-repository.worktribe.com/output/5788891
• Publisher URL: https://link.springer.com/article/10.1007%2Fs00216-021-03430-4