A new view of macula densa cell microanatomy

Gyarmati, Georgina; Shroff, Urvi Nikhil; Riquier-Brison, Anne; Kriz, Wilhelm; Kaissling, Brigitte; Neal, Christopher R.; Arkill, Kenton P.; Ahmadi, Nariman; Gill, Inderbir S.; Moon, Ju-Young; Desposito, Dorinne; Peti-Peterdi, J�nos

Abstract

Although macula densa (MD) cells are chief regulatory cells in the nephron with unique microanatomical features, they have been difficult to study in full detail due to their inaccessibility and limitations in earlier microscopy techniques. The present study used a new mouse model with a comprehensive imaging approach to visualize so far unexplored microanatomical features of MD cells, their regulation, and functional relevance. MD-GFP mice with conditional and partial induction of green fluorescent protein (GFP) expression, which specifically and intensely illuminated only single MD cells, were used with fluorescence microscopy of fixed tissue and live MD cells in vitro and in vivo with complementary electron microscopy of the rat, rabbit, and human kidney. An elaborate network of major and minor cell processes, here named maculapodia, were found at the cell base, projecting toward other MD cells and the glomerular vascular pole. The extent of maculapodia showed upregulation by low dietary salt intake and the female sex. Time-lapse imaging of maculapodia revealed highly dynamic features including rapid outgrowth and an extensive vesicular transport system. Electron microscopy of rat, rabbit, and human kidneys and three-dimensional volume reconstruction in optically cleared whole-mount MD-GFP mouse kidneys further confirmed the presence and projections of maculapodia into the extraglomerular mesangium and afferent and efferent arterioles. The newly identified dynamic and secretory features of MD cells suggest the presence of novel functional and molecular pathways of cell-to-cell communication in the juxtaglomerular apparatus between MD cells and between MD and other target cells. NEW & NOTEWORTHY This study illuminated a physiologically regulated dense network of basal cell major and minor processes (maculapodia) in macula densa (MD) cells. The newly identified dynamic and secretory features of these microanatomical structures suggest the presence of novel functional and molecular pathways of cell-to-cell communication in the juxtaglomerular apparatus between MD and other target cells. Detailed characterization of the function and molecular details of MD cell intercellular communications and their role in physiology and disease warrant further studies.

• Journal Article Type: Article
• Acceptance Date: Jan 18, 2021
• Online Publication Date: Mar 12, 2021
• Publication Date: Mar 1, 2021
• Deposit Date: May 17, 2021
• Publicly Available Date: Mar 2, 2022
• Journal: American Journal of Physiology-Renal Physiology
• Print ISSN: 1931-857X
• Electronic ISSN: 1522-1466
• Publisher: American Physiological Society
• Peer Reviewed: Peer Reviewed
• Volume: 320
• Issue: 3
• Pages: F492-F504
• DOI: https://doi.org/10.1152/ajprenal.00546.2020
• Keywords: Urology; Physiology
• Public URL: https://nottingham-repository.worktribe.com/output/5556626
• Publisher URL: https://journals.physiology.org/doi/full/10.1152/ajprenal.00546.2020