The role of photoprotection in defence of two wheat genotypes against Zymoseptoria tritici

Ajigboye, Olubukola O.; Jayaweera, Dasuni P.; Angelopoulou, Dimitra; Ruban, Alexander V.; Murchie, Erik H.; Pastor, Victoria; Summers, Richard; Ray, Rumiana V.

Abstract

This study provides new insights into the role of photoprotection in preformed and induced defence of two wheat genotypes with contrasting phenotypes to infection by Zymoseptoria tritici. We investigated the mechanisms of the photoprotective response during early infection, including nonphotochemical quenching (NPQ), β-carotene-derived xanthophylls, reactive oxygen species, and the phytohormones abscisic acid (ABA), jasmonic acid (JA), and salicylic acid (SA). Furthermore, we quantified the effects of pathogenesis on photosynthesis, stomatal control, and expression of plant defence molecular markers. The photoprotective mechanism of successful defence involved the qI component of NPQ leading to rapid down-regulation of photosystem II quantum yield and chlorophyll a:b, increased biosynthesis of the xanthophyll neoxanthin and ABA, and the expression of chloroplast-specific enzymes to engage in scavenging of O2●−. Elevated ABA in the resistant genotype correlated with preformed leaf defence traits including low stomatal density, increased expression of wax biosynthesis, and lignification. Z. tritici exhibited reduced germination and branching on the resistant host genotype and hijacked stomatal control in both genotypes by enhancing stomatal sensitivity to light. Increased biosynthesis of JA and anthocyanins, in contrast to SA, were quantified in the incompatible interaction. Our results indicate that ABA and JA in antagonistic action to SA were associated with defence in the resistant genotype, Cougar, against Z. tritici.

Citation

Ajigboye, O. O., Jayaweera, D. P., Angelopoulou, D., Ruban, A. V., Murchie, E. H., Pastor, V., Summers, R., & Ray, R. V. (2021). The role of photoprotection in defence of two wheat genotypes against Zymoseptoria tritici. Plant Pathology, 70(6), 1421-1435. https://doi.org/10.1111/ppa.13392

• Journal Article Type: Article
• Acceptance Date: Apr 12, 2021
• Online Publication Date: May 7, 2021
• Publication Date: 2021-08
• Deposit Date: May 17, 2021
• Publicly Available Date: May 20, 2021
• Journal: Plant Pathology
• Print ISSN: 0032-0862
• Electronic ISSN: 1365-3059
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 70
• Issue: 6
• Pages: 1421-1435
• DOI: https://doi.org/10.1111/ppa.13392
• Keywords: Agronomy and Crop Science; Plant Science; Genetics; Horticulture
• Public URL: https://nottingham-repository.worktribe.com/output/5531023
• Publisher URL: https://bsppjournals.onlinelibrary.wiley.com/doi/10.1111/ppa.13392