Array-based measurements of aero-allergen-specific IgE correlate with skin-prick test reactivity in asthma regardless of specific IgG4 or total IgE measurements

Hamed, Aljali; Todd, Ian; Tighe, Patrick J; Powell, Richard J; Harrison, Tim; Fairclough, Lucy

Abstract

© 2021 Elsevier B.V. Skin prick testing (SPT) and measurement of serum allergen-specific IgE (sIgE) are used to investigate asthma and other allergic conditions. Measurement of serum total IgE (tIgE) and allergen-specific IgG4 (sIgG4) may also be useful. The aim was to ascertain the correlation between these serological parameters and SPT. Sera from 60 suspected asthmatic patients and 18 healthy controls were assayed for sIgE and sIgG4 reactivity against a panel of 70 SPT allergen preparations, and for tIgE. The patients were also assessed by skin prick tests for reactivity to cat, dog, house dust mite and grass allergens. Over 50% of the patients had tIgE levels above the 75th percentile of the controls. 58% of patients and 39% of controls showed sIgE reactivity to ≥1 allergen. The mean number of allergens detected by sIgE was 3.1 in suspected asthma patients and 0.9 in controls. 58% of patients and 50% of controls showed sIgG4 reactivity to ≥1 allergen. The mean number of allergens detected by sIgG4 was 2.5 in patients and 1.7 in controls. For the patients, a strong correlation was observed between clinical SPT reactivity and serum sIgE levels to cat, dog, house dust mite (HDM) and grass allergens. SPT correlations using sIgE/sIgG4 or sIgE/tIgE ratios were not markedly higher. The measurement of serum sIgE by microarray using SPT allergen preparations showed good correlation with clinical SPT reactivity to cat, dog, HDM and grass allergens. This concordance was not improved by measuring tIgE or sIgG4.

Citation

Hamed, A., Todd, I., Tighe, P. J., Powell, R. J., Harrison, T., & Fairclough, L. (2021). Array-based measurements of aero-allergen-specific IgE correlate with skin-prick test reactivity in asthma regardless of specific IgG4 or total IgE measurements. Journal of Immunological Methods, 492, Article 112999. https://doi.org/10.1016/j.jim.2021.112999

• Journal Article Type: Article
• Acceptance Date: Feb 10, 2021
• Online Publication Date: Feb 18, 2021
• Publication Date: May 1, 2021
• Deposit Date: Feb 11, 2021
• Publicly Available Date: Feb 19, 2022
• Journal: Journal of Immunological Methods
• Print ISSN: 0022-1759
• Electronic ISSN: 1872-7905
• Publisher: Elsevier
• Peer Reviewed: Peer Reviewed
• Volume: 492
• Article Number: 112999
• DOI: https://doi.org/10.1016/j.jim.2021.112999
• Keywords: Allergic asthma; aero-allergen; IgG4; IgE; Microarray; skin prick test
• Public URL: https://nottingham-repository.worktribe.com/output/5316837
• Publisher URL: https://linkinghub.elsevier.com/retrieve/pii/S0022175921000442