Detection of genome-edited and endogenously expressed G protein-coupled receptors

Soave, Mark; Stoddart, Leigh A.; White, Carl W.; Kilpatrick, Laura E.; Goulding, Jo�lle; Briddon, Stephen J.; Hill, Stephen J.

Abstract

© 2021 The Authors. The FEBS Journal published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. G protein-coupled receptors (GPCRs) are the largest family of membrane receptors and major targets for FDA-approved drugs. The ability to quantify GPCR expression and ligand binding characteristics in different cell types and tissues is therefore important for drug discovery. The advent of genome editing along with developments in fluorescent ligand design offers exciting new possibilities to probe GPCRs in their native environment. This review provides an overview of the recent technical advances employed to study the localisation and ligand binding characteristics of genome-edited and endogenously expressed GPCRs.

Citation

Soave, M., Stoddart, L. A., White, C. W., Kilpatrick, L. E., Goulding, J., Briddon, S. J., & Hill, S. J. (2021). Detection of genome-edited and endogenously expressed G protein-coupled receptors. FEBS Journal, 288(8), 2585-2601. https://doi.org/10.1111/febs.15729

• Journal Article Type: Article
• Acceptance Date: Jan 25, 2021
• Online Publication Date: Jan 28, 2021
• Publication Date: 2021-04
• Deposit Date: Feb 18, 2021
• Publicly Available Date: Feb 18, 2021
• Journal: FEBS Journal
• Print ISSN: 1742-464X
• Electronic ISSN: 1742-4658
• Publisher: Wiley
• Peer Reviewed: Peer Reviewed
• Volume: 288
• Issue: 8
• Pages: 2585-2601
• DOI: https://doi.org/10.1111/febs.15729
• Keywords: Cell Biology; Biochemistry; Molecular Biology
• Public URL: https://nottingham-repository.worktribe.com/output/5276190
• Publisher URL: https://febs.onlinelibrary.wiley.com/doi/10.1111/febs.15729
• Additional Information: Received: 2020-10-28; Accepted: 2021-01-25; Published: 2021-01-28