Dr TANIA MENDONCA Tania.Mendonca@nottingham.ac.uk
RESEARCH FELLOW
Dr TANIA MENDONCA Tania.Mendonca@nottingham.ac.uk
RESEARCH FELLOW
Roman Urban
Kellie Lucken
George Coney
Neil M. Kad
Manlio Tassieri
Professor AMANDA WRIGHT Amanda.Wright@nottingham.ac.uk
PROFESSOR OF OPTICS
Dr Daniel Booth DANIEL.BOOTH@NOTTINGHAM.AC.UK
PRINCIPAL RESEARCH FELLOW
Dr TANIA MENDONCA Tania.Mendonca@nottingham.ac.uk
Researcher
Roman Urban
Researcher
Kellie Lucken
Researcher
George Coney
Researcher
Neil Kad
Project Member
Manlio Tassieri
Project Member
Professor AMANDA WRIGHT Amanda.Wright@nottingham.ac.uk
Project Member
Dr Daniel Booth DANIEL.BOOTH@NOTTINGHAM.AC.UK
Project Leader
In dividing cells, chromosomes are coated in a sheath of proteins and RNA called the mitotic chromosome periphery. This sheath is thought to confer biophysical properties to chromosomes, critical for successful cell division. However, the details of chromosome mechanics, and specifically, if and how the chromosome periphery contributes to them, remain poorly understood. In this study, we present a comprehensive characterisation of single-chromosome mechanics using optical tweezers and an improved broadband microrheology analysis. We extend this analysis to direct measurements of the chromosome periphery by manipulating levels of Ki-67, its chief organiser, and apply a rheological model to isolate its contribution to chromosome mechanics. We report that the chromosome periphery governs dynamic self-reorganisation of chromosomes and acts as a structural constraint, providing force-damping properties. This work provides significant insight into chromosome mechanics and will inform our understanding of the mitotic chromosome periphery’s role in cell division.
Mendonca, T., Urban, R., Lucken, K., Coney, G., Kad, N. M., Tassieri, M., Wright, A. J., & Booth, D. G. (2025). The mitotic chromosome periphery modulates chromosome mechanics. Nature Communications, 16, Article 6399. https://doi.org/10.1038/s41467-025-61755-5
Journal Article Type | Article |
---|---|
Acceptance Date | Jun 27, 2025 |
Online Publication Date | Jul 10, 2025 |
Publication Date | Jul 10, 2025 |
Deposit Date | Jul 15, 2025 |
Publicly Available Date | Jul 16, 2025 |
Journal | Nature Communications |
Electronic ISSN | 2041-1723 |
Publisher | Nature Publishing Group |
Peer Reviewed | Peer Reviewed |
Volume | 16 |
Article Number | 6399 |
DOI | https://doi.org/10.1038/s41467-025-61755-5 |
Public URL | https://nottingham-repository.worktribe.com/output/51610376 |
Publisher URL | https://www.nature.com/articles/s41467-025-61755-5# |
s41467-025-61755-5
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Copyright Statement
Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder.
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